ペパーミント(Mentha piperita L.)のシュート培養における生育と香気成分及びコーヒー酸誘導体生産

概要

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Axenic shoot culture of Mentha piperita was established from young shoots of plants cultivated in a field, and essential oil and phenolic production of M. piperita shoots cultured on Murashige-Skoog (MS) solid medium was investigated. The main compound of M. piperita cultivated in a field is normaly menthol, but carvone was detected as a main compound in the shoots cultured on phytohormone-free MS solid medium under 16h/day light for 6 weeks. In contrast menthol was not detected by GC-MS analysis in the shoot culture. M. piperita shoots cultured on phytohormone-free MS solid medium in the dark for 6 weeks produced only carvone. M. piperita plantlets obtained by culturing in vitro for 3 weeks were transplanted to soil, and essential oil was examined. Carvone was detected as a main compound during cultivation period (until 7 weeks after transplantation), while production of menthol started at week 3 after the transplantation. Rosmarnic acid (RA), caffeic acid derivative, was detected in M. piperita shoots cultured on MS solid medium under 16h/day light and in the dark, but other polyphenols such as lithospermic acid and lithospermic acid B were not detected by HPLC analysis. Potted plants, regenerated from shoot cultures, accumulated RA (ca. 4%) at a relatively high level as a main compound after 7 weeks of cultivation.
日本食品化学学会の論文
1998-11-30