PAP2遺伝子による植物ポリフェノール代謝制御 : コミカンソウのフェノール類生産

概要

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Phyllanthus urinaria hairy root integrated PAP2 (production of anthocyanin pigment) gene was derived using Agrobacterium rhizogenes. In the PAP2 gene-integrated-hairy root cultures, phenylpropanoid derivative (1-O-p-coumaryl-β-D-glucopyranose) and anthocyanins (e.g. cyanidin 3-O-glucoside) were accumulated in higher concentrations compared to those of normal type (PAP2 gene not integrated) hairy root. This observation shows a possibility of PAP2 gene utilization for the development of polyphenol rich plants being useful for functional food resource. In the shoot tissues of P. urinaria, large amount of hydrolysable type tannins (geraniin) was observed. On the other hand, in the root tissues, proanthocyanidin type tannins ((-)-epigallocatechin, (-)-epicatechin, (-)-epigallocatechin 3-O-gallate and (-)-epicatechin 3-O-gallate) were produced with high concentrations. This tissue specific accumulation of hydrolysable and proanthocyanidin type tannins was similar to that of P. niruri L.. P. urinaria seemed to be an useful resource plant for functional polyphenol compounds mentioned above.
2010-04-14