Hemiphragma heterophyllumの組織培養と二次代謝物生産

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Shoot culture of Hemiphragma heterophyllum (Scrophulariaceae) was established for the first time. Six known compounds, plantamajoside, hemiphroside B, 2', 6"-O-diacetylplantamajoside, globularin, iso-scrophurarioside and cinnamic acid were isolated from the shoot tissues of H. heterophyllum. 2', 6"-O-diacetylplantamajoside, which was isolated from this genius for the first time, is important chemotaxonomic marker suggesting the relationship between Hemiphragma and Wulfenia. Hairy root culture of H. heterophyllum was also established. In the hairy root cultures, high concentration of plantamajoside and hemiphroside B was observed. A phenolic compound p-tyrosol, which was not detected in the parent plantlets, was also isolated from the hairy roots. H. heterophyllum has a potential to become a new useful material for functional secondary metabolites in the field of food ingredients.
2006-05-08