Mechanism for Interference of Ethinamate in Zimmermann Test for Determination of Urinary 17-Ketosteroids
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概要
- 論文の詳細を見る
The mechanism by which dosage of ethinamate elevates falsely the values for the urinary 17-ketosteroids has been investigated. A specimen of urine collected following oral administration of ethinamate to man was hydrolyzed with sulfuric acid and then extracted with dichloromethane. From the urinary extract two non-steroidal chromogens responsible for the Zimmermann reaction were isolated. The spectral and chromatographic properties indicated that these chromogens would be 1-acetylcyclohex-1-en-4-ol and 1-acetylcyclohex-1-en-5-ol. Their structures were unequivocally characterized by comparison with the synthetic samples. It has been disclosed that during the acid-catalyzed hydrolysis of the conjugates in the process of 17-ketosteroids determination the 4- and 3-hydroxylated metabolites formed from ethinamate are converted artificially into the ketones which give a typical color for the 17-ketosteroids in the Zimmermann reaction.
- 公益社団法人日本薬学会の論文
- 1975-10-25
著者
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島田 和武
東北大学薬学部
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島田 和武
Pharmaceutical Institute, Tohoku University
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南原 利夫
Pharmaceutical Institute Tohoku University
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島田 和武
Faculty Of Pharmaceutical Sciences Kanazawa University
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花渕 玲子
Pharmaceutical Institute, Tohoku University
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花渕 玲子
Pharmaceutical Institute Tohoku University
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