The Preparation of Steroid N-Hydroxysuccinimide Esters and Their Reactivities with Bovine Serum Albumin

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概要

• 論文の詳細を見る

• The N-hydroxysuccinimide esters of testosterone and cortisol were prepared from the corresponding carboxylic acid derivatives by a carbodiimide method. These activated esters were covalently linked to bovine serum albumin in organic solvent/phosphate buffer (pH 7.4) employing various molar ratios of the steroid to protein. The results indicated that the activated ester method would be useful for enzyme labeling in enzyme immunoassay of steroids in respects of simplicity and reproducibility.

• 社団法人日本薬学会の論文
• 1979-03-25

著者

• 宮入 伸一

  Pharmaceutical Institute Tohoku University

• 宮入 伸一

  東北大学薬学部

• 南原 利夫

  Pharmaceutical Institute, Tohoku University

• 石井 和夫

  School of Health Sciences, Kyorin University

• 細田 宏

  Pharmaceutical Institute Tohoku University

• 吉田 浩子

  Pharmaceutical Institute Tohoku University

• 酒井 康夫

  Tokyo Laboratory Daiichi Pure Chemicals Co. Ltd.

• 酒井 康夫

  Pharmaceutical Institute, Tohoku University

• 石井 和夫

  Pharmaceutical Institute, Tohoku University

• 南原 利夫

  Pharmaceutical Institute Tohoku University

• 石井 和夫

  杏林大学保健学部

• 石井 和夫

  Pharmaceutical Institute Tohoku University

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