Specificity of Antiserum raised against Estetrol using 6-O-Carboxymethyloxime-Bovine Serum Albumin Conjugate
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概要
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In order to obtain the specific antiserum used for radioimmunoassay of estetrol a new hapten-carrier conjugate was prepared from 6-ketoestetrol O-carboxymethyloxime by coupling with bovine serum albumin employing the mixed anhydride technique. The specificity of anti-estetrol antiserum elicited in the rabbit by immunization with this antigen was tested by cross-reaction studies with the closely related steroids. The results indicated that highly specific antiserum to estetrol would be produced by antigen whose steroidal moiety is coupled to a protein through the position remote from the inherent functional groups.
- 社団法人日本薬学会の論文
- 1976-07-25
著者
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南原 利夫
Pharmaceutical Institute, Tohoku University
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沼澤 光輝
Pharmaceutical Institute Tohoku University
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土田 由紀子
Department of Microbiology, Hoshi College of Pharmacy
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田中 東一
Pharmaceutical Institute Tohoku University
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土田 由紀子
Pharmaceutical Institute, Tohoku University
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南原 利夫
Pharmaceutical Institute Tohoku University
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土田 由紀子
Department Of Microbiology Hoshi College Of Pharmacy
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