Specificity of Antisera raised against Cortisol-4-Bovine Serum Albumin Conjugates in Radioimmunoassay
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概要
- 論文の詳細を見る
In order to obtain specific antisera for use in radioimmunoassay and enzyme immunoassay of cortisol, new hapten-carrier conjugates were prepared from 4-hemisuccinoyloxycortisol, 4-(carboxymethylthio) cortisol, 4-(2-carboxyethylthio) cortisol and 4-(2-hemisuccinoyloxyethylthio) cortisol by coupling with bovine serum albumin (BSA) employing the N-hydroxysuccinimide ester method or mixed anhydride method. The specificity of anti-cortisol antisera elicited in rabbits by immunization with these antigens was tested by cross-reaction studies with closely related steroids and by measuring the amount of cortisol in urine specimens by means of radioimmunoassay. Comparison of the specificity of these antisera with that of other antisera prepared with BSA conjugates of cortisol 3-(O-carboxymethyl) oxime and 21-hemisuccinoylcortisol was also carried out. The results showed that the antisera obtained were sufficiently specific and thus that the C-4 position was suitable for the attachment of a carrier protein for use in the production of antibodies.
- 社団法人日本薬学会の論文
- 1980-11-25
著者
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宮入 伸一
Pharmaceutical Institute Tohoku University
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宮入 伸一
東北大学薬学部
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南原 利夫
Pharmaceutical Institute, Tohoku University
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細田 宏
Pharmaceutical Institute Tohoku University
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小林 典裕
Pharmaceutical Institute Tohoku University
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南原 利夫
Pharmaceutical Institute Tohoku University
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