Radioimmunoassay of 11-Deoxycortisol. Specificity of Antisera raised against 11-Deoxycortisol-[C-4]-Bovine Serum Albumin Conjugates
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概要
- 論文の詳細を見る
In order to obtain specific antisera for use in immunoassays of 11-deoxycortisol, new hapten-carrier conjugates were prepared from 4-hemisuccinoyloxy-11-deoxycortisol, 4-(carboxymethylthio)-11-deoxycortisol, 4-(2-carboxyethylthio)-11-deoxycortisol and 4-(2-hemisuccinoyloxyethylthio)-11-deoxycortisol by coupling with bovine serum albumin employing the N-succinimidyl ester method. The specificity of anti-11-deoxycortisol antisera elicited in rabbits by immunization with these antigens was tested by crossreaction studies with closely related steroids and by measuring the amount of 11-deoxycortisol in plasma specimens by means of radioimmunoassay. The results showed that the antisera obtained were reasonably specific and useful for the determination of plasma 11-deoxycortisol levels in metyrapone tests. The assay can be done on methylene chloride extracts of plasma.
- 社団法人日本薬学会の論文
- 1982-06-25
著者
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宮入 伸一
Pharmaceutical Institute Tohoku University
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宮入 伸一
東北大学薬学部
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南原 利夫
Pharmaceutical Institute, Tohoku University
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細田 宏
Pharmaceutical Institute Tohoku University
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小林 典裕
Pharmaceutical Institute Tohoku University
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南原 利夫
東北大学医学部薬学科
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南原 利夫
Pharmaceutical Institute Tohoku University
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