AN ENZYME IMMUNOASSAY METHOD WITH A MONOCLONAL ANTIBODY FOR THE DETERMINATION OF 11-DEOXYCORTISOL
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概要
- 論文の詳細を見る
An enzyme immunoassay method using a monclonal antibody for the determination of 11-deoxycortisol is described. The anti-11-deoxycortisol antibody was produced in ascites by inoculating antibody-secreting cells into BALB/c mice. The N-succinimidyl esters of carboxylated steroids were treated with β-galactosidase to give enzyme-labeled antigens. The bound and free fractions were separated by a double antibody-protein A method. It was found that the monoclonal antibody had a high affinity for a homologous enzyme-labeled antigen prepared from 4-(2-carboxyethylthio)-11-deoxycortisol and the binding was inhibited by the analyte 11-deoxycortisol, resulting in a satisfactory assay sensitivity.
- 社団法人日本薬学会の論文
- 1985-01-25
著者
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南原 利夫
Pharmaceutical Institute, Tohoku University
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沢田 純一
国立衛試
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寺尾 允男
National Institute of Hygienic Sciences
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細田 宏
Pharmaceutical Institute Tohoku University
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小林 典裕
Pharmaceutical Institute Tohoku University
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寺尾 允男
国立衛生試験所
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寺尾 允男
National Institute Of Health Sciences
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田村 早樹子
Pharmaceutical Institute, Tohoku University
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沢田 純一
National Institute of Hygienic Sciences
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南原 利夫
東北大学医学部薬学科
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南原 利夫
Pharmaceutical Institute Tohoku University
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田村 早樹子
Pharmaceutical Institute Tohoku University
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