Syntheses of New Haptens for Radioimmunoassay of Estradiol
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概要
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In order to obtain much more specific antiserum required for radioimmunoassay of estradiol, the preparation of new promising haptens has been undertaken. First, the C-6 epimeric 6-hydroxyestradiol 3,17-bis (tetrahydropyranyl) ethers (VIII, XI) were treated with succinic anhydride and pyridine in the usual manner, respectively. Subsequent removal of the protecting groups provided the desired 6-hydroxyestradiol 6-hemisuccinates (X, XIII). Secondly, 7α-hydroxyestradiol 7-hemisuccinate (XXIII) has been also synthesized from 6-dehydroestradiol (XIV) by way of the 6α, 7α-epoxide (XVIII) as a key intermediate. Reductive cleavage of the 6,7-oxido ring with metal hydride yielded solely the 7α-hydroxyl compound (XIX). Transformation into the 7-hemisuccinate (XXI) followed by elimination of the protecting group afforded XXIII in a satisfactory yield.
- 社団法人日本薬学会の論文
- 1974-05-25
著者
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南原 利夫
Pharmaceutical Institute, Tohoku University
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沼澤 光輝
Pharmaceutical Institute Tohoku University
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高橋 正樹
Pharmaceutical Institute, Tohoku University
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南原 利夫
Pharmaceutical Institute Tohoku University
-
高橋 正樹
Pharmaceutical Institute Tohoku University
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