A Direct Enzyme Immunoassay of 6β-Hydroxycortisol in Human Urine
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概要
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A sensitive enzyme immunoassay for urine 6β-hydroxycortisol has been developed. Enzyme labeling of 6β-hydroxycortisol was accomplished by the N-succinimidyl ester method. The use of N-succinimidyl ester of 6β-hydroxycortisol 21-hemisuccinate and β-galactosidase in an appropriate molar ratio provided a conjugate suitable for enzyme immunoassay. Antiserum was prepared by immunization with the 6β-hydroxycortisol 21-hemisuccinate-bovine serum albumin conjugate in the rabbit. Sufficient sensitivity and improved specificity of the assay system could be obtained by the selective blocking of less specific antibodies. The quantitation limit of 6β-hydroxycortisol was approximately 10 pg, which is comparable to that of radioimmunoassay. The intra- and inter-assay coefficients of variation for 6β-hydroxycortisol in human urine were 5.9-8.1% and 2.8-12.3%, respectively.
- 公益社団法人日本薬学会の論文
- 1981-01-25
著者
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南原 利夫
Pharmaceutical Institute, Tohoku University
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細田 宏
Pharmaceutical Institute Tohoku University
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酒井 康夫
Tokyo Laboratory Daiichi Pure Chemicals Co. Ltd.
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酒井 康夫
Pharmaceutical Institute, Tohoku University
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南原 利夫
東北大学医学部薬学科
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南原 利夫
Pharmaceutical Institute Tohoku University
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