Bound/Free Separation Methods in Steroid Enzyme Immunoassay with Monoclonal Antibody

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概要

• 論文の詳細を見る

• Methods for separating bound and free fractions in steroid enzyme immunoassay with a monoclonal antibody are described. Three liquid-phase separation techniques and related preincubation methods were studied using β-galactosidase and horseradish peroxidase as labels. In the double- and triple-antibody systems, the effects of concentrations of first, second and third antibodies, including anti-immunoglobulin G Fc fragment antisera, and incubation times on immune precipitation were examined. Differences between the two enzyme labels in immunoreactivity were observed in some cases; this can be explained in terms of a steric interaction. Under optimal conditions, a dose-response curve with a high sensitivity was obtained in each 11-deoxycortisol assay system.

• 公益社団法人日本薬学会の論文
• 1988-09-25

著者

• 南原 利夫

  Pharmaceutical Institute, Tohoku University

• 細田 宏

  Pharmaceutical Institute Tohoku University

• 田村 早樹子

  Pharmaceutical Institute, Tohoku University

• 塚本 玲子

  Pharmaceutical Institute, Tohoku University

• 南原 利夫

  東北大学医学部薬学科

• 南原 利夫

  Pharmaceutical Institute Tohoku University

• 塚本 玲子

  Pharmaceutical Institute Tohoku University

• 田村 早樹子

  Pharmaceutical Institute Tohoku University

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