Effect of CAWS, a Mannoprotein-β-glucan Complex of Candida albicans, on Leukocyte, Endothelial Cell, and Platelet Functions in Vitro
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概要
- 論文の詳細を見る
Candida albicans is a medically important fungus which induces a disseminated candidasis and candidemia in immunocompromised hosts, and releases a polysaccharide fraction into the blood. We recently found that C. albicans released a water-soluble polysaccharide fraction (CAWS) into synthetic medium and demonstrated that CAWS was mainly composed of a complex of mannan and β-glucan. In the murine system, CAWS showed a lethality resembling anaphylactic shock when administered i.v., and induced coronary arteritis similar to Kawasaki Disease (KD) when given i.p. In the present study, we examined the biological activity of CAWS in the cell culture and found the following: i) CAWS slightly induced production of IFN-γ and IL-6 by splenocytes at lower dose (ca. 10μg/ml), but at a higher dose strongly inhibited the proliferation of splenocytes induced by a B cell mitogen, lipopolysaccharide (LPS) and a T cell mitogen, concanavalin A. ii) The viability of these splenocytes monitored by propidium iodide staining was significantly reduced. iii) The addition of CAWS to a culture of monophage RAW264.7 cells significantly reduced cellular growth rate dose dependently. iv) The LPS-mediated synthesis of cytokines by RAW264.7 cells was significantly inhibited by CAWS. v) CAWS induced an aggregation of platelets in human platelet-rich plasma, and vi) CAWS inhibited the production of thrombomodulin by human umbilical endothelial cells and acted synergistically with TNF-α. Thus, CAWS strongly inhibited the cellular functions of leukocytes in vitro, partly through direct cytotoxicity. The enhanced production in injured cells of the vascular endothelium would be related to the local inflammatory response in the coronary artery.
- 社団法人日本薬学会の論文
- 2003-02-01
著者
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OHNO NAOHITO
Tokyo College of Pharmacy
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Ohno Naohito
東京薬科大学 免疫学
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Ohno Naohito
東京薬科大学 薬 微生物
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Ohno Naohito
Lab. For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo Univ. Of Pharmacy And Lif
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Ohno N
Laboratory For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm
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Miura N
Laboratory For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm
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Nagi Miura
Laboratory For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm
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Adachi Yoshiyuki
東京薬科大学 免疫学
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Adachi Y
Tokyo Univ. Pharmacy And Life Sci. Tokyo Jpn
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KURIHARA Kiyoshi
Tokyo University of Pharmacy and Life Science, School of Pharmacy
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SHINGO Yuko
Tokyo University of Pharmacy and Life Science, School of Pharmacy
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MIURA Noriko
Tokyo University of Pharmacy and Life Science, School of Pharmacy
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HORIE Shuichi
Department of Clinical Biochemistry, Faculty of Pharmaceutical Sciences, Teikyo University
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USUI Yukio
Aoba University
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ADACHI Yoshiyuki
Tokyo University of Pharmacy and Life Science, School of Pharmacy
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YADOMAE Toshiro
Tokyo University of Pharmacy and Life Science, School of Pharmacy
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Usui Y
Aoba University
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Shingo Yuko
Laboratory For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm
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Adachi Yoshiyuki
Laboratory For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm
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Ohno Naohito
Laboratory Of Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharma
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Ohno Naohito
Laboratory For Immunopharmacology Of Microbial Products Tokyo University Of Pharmacy And Life Scienc
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Kurihara Kiyoshi
Laboratory For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm
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Horie Syunji
Laboratory Of Pharmacology Faculty Of Pharmaceutical Sciences Josai International University
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Horie S
Laboratory Of Chemical Pharmacology Graduate School Of Pharmaceutical Sciences Chiba University
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Horie S
Department Of Clinical Biochemistry Faculty Of Pharmaceutical Sciences Teikyo University
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Horie Shuichi
Department Of Clinical Biochemistry Faculty Of Pharmaceutical Sciences Teikyo University
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Yadomae T
Laboratory For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm
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Yadomae Toshiro
Tokyo College Of Pharmacy
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Ohno Naohito
Laboratory For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm
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