Immunochemical Characterization of Alkaline-soluble Polysaccharide, P-1, from the Kernels of Prunus mume SIEB. et ZUCC.

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概要

• 論文の詳細を見る

• Polyclonal antibodies against P-1, a pectic polysaccharide fraction extracted with 0.5M NaOH from the kernels of Prunus mume and consisted of arabino-galacturonan, and I-3, the partial acid (0.1M trifluoroacetic acid) hydrolysate of P-1, were prepared in Japanese white rabbits. Competitive ELISA experiments strongly suggested that anti P-1 and anti I-3 antibodies were different but P-1 and I-3 cross-reacted with each other to recognize a partly similar epitope structure. The reactivities of polysaccharide fractions from the raw flesh of P. mume, and the kernels of apricot and peach extracted with either water or sodium hydroxide were examined using both antisera by the indirect competitive ELISA method. The polysaccharide fractions extracted with sodium hydroxide Solutions had the reactivities but not those extracted with cold and hot water. These facts suggested that the similar structure of polysaccharides to P-1 was present in the flesh of P. mume and the kernels of apricot and peach. However, neither pectin of apple nor citrus had reactivity with each antiserum. P-1 would be different in chemical structure from a commercially available pectin, a Water-soluble polysaccharide from apple and citrus.

• 社団法人日本農芸化学会の論文
• 1996-07-23

著者

• Ohno Naohito

  東京薬科大学 免疫学

• Ohno Naohito

  東京薬科大学 薬 微生物

• Ohno Naohito

  Lab. For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo Univ. Of Pharmacy And Lif

• Ohno N

  Laboratory For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm

• Ohno Naohito

  Laboratory Of Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharma

• Ohno Naohito

  Laboratory For Immunopharmacology Of Microbial Products Tokyo University Of Pharmacy And Life Scienc

• Ohno Naohito

  School Of Pharmacy Tokkyo University Of Pharmacy And Life Science

• DOGASAKI Chikaku

  Faculty of Environmental Health Science, Azabu University

• NISHIJIMA Motohiro

  Tokyo Metropolitan Research Laboratory of Public Health

• YADOMAE Toshiro

  School of Pharmacy, Tokyo University of Pharmacy and Life Science

• MIYAZAKI Toshio

  School of Pharmacy, Tokyo University of Pharmacy and Life Science

• NISHIJIMA Masahiro

  Department of Biochemistry and Cell Biology, National Institute of Health

• Miyazaki T

  Department Of Applied Microbiology Nippon Roche Research Center

• Miyazaki Toshio

  School Of Pharmacy Tokyo University Of Pharmacy And Life Science

• Nishijima Masahiro

  Department Of Biochemistry And Cell Biology National Institute Of Health

• Yadomae T

  Laboratory For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm

• Dogasaki C

  Azabu Univ. Kanagawa Jpn

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