Dissociation of Toll-Like Receptor 2-Mediated Innate Immune Response to Zymosan by Organic Solvent-Treatment without Loss of Dectin-1 Reactivity(Molecular and Cell Biology)
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概要
- 論文の詳細を見る
Zymosan activates phagocytes through the innate immune system and causes inflammatory responses in animals. Because of the complexity of the active substances included in Zymosan preparations, simplifying the active moiety actually responsible for innate immune recognition is needed. One way to remove possible active substances from commercially available Zymosan preparations is to wash then with pyrogen-free water to obtain a ZWIS (Zymosan water insoluble fraction), ethanol insoluble (EtIS), or chloroform/methanol insoluble (CMIS) preparation. The effects of various washed Zymosan preparations on nuclear factor (NF)-κB activation and binding to β-glucan recognition protein were examined. Significant NF-κB activation by Toll-like receptor (TLR) 2-expressing HEK293 cells and enhanced NF-κB activity via the co-expression of TLR2 and Dectin-1, a functional β-glucan receptor, was only observed in response to ZWIS. However, the ability of Zymosan preparations to bind Dectin-1 protein was not altered even after treatment with the organic solvents by which the TLR2-mediated NF-κB activity was abolished. Another NF-κB activation pathway involving CARD9/Bcl10 was triggered by these Zymosan preparations in the presence of Dectin-1. The results suggest that the β-glucan-dependent characteristics of Zymosan were not affected by the washing with chloroform/methanol or ethanol, and that TLR2-mediated activity was easily eliminated with these organic solvents. This treatment might be useful for distinguishing natural ligands for TLR2 and β-glucan receptors when studying the innate immune response to fungal macromolecules.
- 社団法人日本薬学会の論文
- 2008-01-01
著者
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三浦 典子
東京薬科大学免疫学
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TAMURA Hiroshi
Seikagaku Biobusiness Corporation
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Manabe Noriyoshi
International Clinical Research Center Research Institute International Medical Center Of Japan
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ADACHI Yoshiyuki
Laboratory far Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pha
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OHNO Naohito
Laboratory far Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pha
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Ohno Naohito
東京薬科大学 免疫学
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Ohno Naohito
東京薬科大学 薬 微生物
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Ohno Naohito
Lab. For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo Univ. Of Pharmacy And Lif
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Ohno N
Laboratory For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm
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Miura N
Laboratory For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm
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Nagi-miura Noriko
東京薬科大学 薬学部免疫学
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Nagi Miura
Laboratory For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm
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Adachi Yoshiyuki
東京薬科大学 免疫学
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Adachi Y
Tokyo Univ. Pharmacy And Life Sci. Tokyo Jpn
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Adachi Yoshiyuki
Laboratory Far Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm
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IKEDA Yoshihiko
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pha
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ISHII Takashi
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pha
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MIURA Noriko
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pha
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Ikeda Yoshihiko
Laboratory For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm
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Adachi Yoshiyuki
Laboratory For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm
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Ohno Naohito
Laboratory Of Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharma
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Ohno Naohito
Laboratory For Immunopharmacology Of Microbial Products Tokyo University Of Pharmacy And Life Scienc
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Miura Noriko
Laboratory For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm
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Ohno Naohito
Tokyo College Of Pharmacy:the Tokyo Metropolitan Research Laboratory Of Public Health
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Ohno N
Laboratory Of Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharma
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Tamura H
Seikagaku Corporation
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Harada Toshie
Seikagaku Corporation
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Adachi Yoshiyuki
Laboratory For Immunopharmacology Of Microbial Products Tokyo University Of Pharmacy And Life Scienc
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Nagi Miura
Laboratory For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm
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Ishii Takashi
Laboratory For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm
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Ohno Naohito
Laboratory For Immunopharmacology Of Microbial Products School Of Pharmacy Tokyo University Of Pharm
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