Enhancement of de Novo Fatty Acid Biosynthesis in Hepatic Cell Line Huh7 Expressing Hepatitis C Virus Core Protein(Biochemistry)
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概要
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Hepatitis C virus (HCV) core protein plays important roles in the pathogeneses of liver steatosis as well as hepatocellular carcinomas due to HCV infection. In this study, we examined de novo fatty acid biosynthesis in hepatic cell line Huh7 cells expressing HCV core protein. The rate of metabolic labeling of cellular fatty acids with [^3H] acetate in core-expressing (Uc39-6) cells was ca. 1.5-fold higher than that in non-expressing (Uc321) cells. The enzyme activities responsible for fatty acid biosynthesis were assayed in vitro. Cytosolic acetyl-CoA carboxylase activity in Uc39-6 cells was ca. 1.6-fold higher than that in Uc321 cells. On the other hand, cytosolic fatty acid synthase activity in Uc39-6 cells was only slightly higher than that in Uc321 cells. Imnmnoblot analysis of acetyl-CoA carboxylase 1 (ACC1), which is a rate-limiting enzyme for fatty acid biosynthesis, revealed a higher expression level of the protein in Uc39-6 cells than in Uc321 cells. The ACC1 mRNA content in Uc39-6 cells was 1.4-fold higher than that in Uc321 cells. These results strongly suggest that enhancement of fatty acid biosynthesis in core-expressing cells is caused by increased expression of fatty acid biosynthetic enzymes, especially ACC1. Up-regulation of de novo fatty acid biosynthesis by HCV core protein may affect cellular lipid metabolism, resulting in neutral lipid accumulation in HCV-infected cells.
- 公益社団法人日本薬学会の論文
- 2006-09-01
著者
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Tanaka Yasuhito
Department Of Orthopaedic Surgery Nara Medical University
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Fukasawa Masayoshi
Department of Health Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo
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HANADA Kentaro
Department of Biochemistry and Cell Biology, Laboratory of Biomembranes, National Institute of Infec
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MIYAMURA TATSUO
Department of Virology II, National Institute of Infectious Diseases
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Hanada Kentaro
Department Of Biochemistry And Cell Biology National Institute Of Infectious Diseases
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NISHIJIMA Masahiro
Department of Biochemistry and Cell Biology, National Institute of Health
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SATO Shigeko
Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases
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SUZUKI Tetsuro
Department of Virology II, National Institute of Infectious Diseases
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Sato Shigeko
Department Of Biochemistry And Cell Biology National Institute Of Infectious Diseases
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Nitahara-kasahara Yuko
Department Of Biochemistry And Cell Biology National Institute Of Infectious Diseases
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Suzuki Tetsuro
Department Of Virology Ii National Institute Of Infectious Diseases
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Nishijima Masahiro
Department Of Biochemistry And Cell Biology National Institute Of Health
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Miyamura Tatsuo
Department Of Virology Ii National Institute Of Infectious Diseases
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ONO Yujin
Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases
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Fukasawa Masayoshi
Department Of Biochemistry And Cell Biology National Institute Of Infectious Diseases
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Ono Yujin
Department Of Biochemistry And Cell Biology National Institute Of Infectious Diseases
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Suzuki Tetsuro
Department Of Physics Faculty Of Science Tokyo University:(present)the Toshiba Transistor Plant
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Tanaka Yasuhito
Department Of Biochemistry And Cell Biology National Institute Of Health
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SUZUKI Tetsuro
Department of Electrical and Information Engineering, Yamagata University
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