Delayed Release of Prostaglandins from Arachidonic Acid and Kinetic Changes in Prostaglandin H Synthase Activity on the Induction of Prostaglandin H Synthase-2 after Lipopolysaccharide-Treatment of RAW264.7 Macrophage-Like Cells
スポンサーリンク
概要
- 論文の詳細を見る
In a mouse macrophage-like cell line, RAW264.7,arachidonic acid was cleaved within 30 min of lipopolysaccharide (LPS)-treatment. However, prostaglandin (PG) synthesis did not accompany this cleavage, being delayed by 4h, although significant PGH synthase (PGHS) activity was detected in the lysate of LPS-nontreated cells. The K_m value of this basal PGHS activity toward arachidonic acid was more than one hundred-fold higher than that for the lysate of cells treated with LPS for 4h. Changes in the sensitivity of the PGHS activity to nonsteroidal antiinflammatory drugs after LPS-treatment also suggested induction of PGHS with different properties from that in the case of basal PGHS. The concomitant increase in PGH synthase (PGHS) activity with the induction of PGHS-2 protein after LPS-treatment suggested a contribution by PGHS-2 to the delayed synthesis of PGs in LPS-treated macrophage cells. As for PGHS in the control cells without LPS-treatment, a different K_m value from that of PGHS-1 and the lack of cross-reactivity to anti-PGHS-1 antibodies suggested that this basal PGHS was different from the typical PGHS-1. The lower affinity of this enzyme for arachidonic acid might be the reason for the failure to release PGs by the cells without LPS-treatment.
- 公益社団法人日本薬学会の論文
- 1997-04-15
著者
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Tanaka Yasuhito
Department Of Orthopaedic Surgery Nara Medical University
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TAKAHASHI Miyuki
Department of Biotechnological Science, Faculty of Biology-Oriented Science and Technology, Kinki Un
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Amano Fumio
Department Of Biochemistry And Cell Biology National Institute Of Infectious Diseases
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KAWAGUCHI Mayumi
Department of Pharmacy, Ogaki Municipal Hospital
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Takahashi Miyuki
Department Of Biochemistry And Cell Biology National Institute Of Health
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Tanaka Y
Toho Univ. School Of Pharmaceutical Sci. Chiba Jpn
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Kawaguchi Mayumi
Department Of Biochemistry And Cell Biology National Institute Of Health
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Amano Fumio
Department Of Biochemistry And Cell Biology National Institute Of Health
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Tanaka Yasuhito
Department Of Biochemistry And Cell Biology National Institute Of Health
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Takahashi Miyuki
Department of Applied Science, Faculty of Technology, Tokyo Denki University
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