Mechanism of Resistance to Oxidative Stress in Doxorubicin Resistant Cells

スポンサーリンク

概要

• 論文の詳細を見る

• Doxorubicin (DOX) is an anthracycline drug widely used in chemotherapy for cancer patients, but it often gives rise to multidrug resistance in cancer cells. The purpose of this work was to study the effect of hydrogen peroxide in DOX-sensitive mouse P388/S leukemia cells and in the DOX-resistant cell line. Hydrogen peroxide induced a significant increase in dose- and time-response cell death in cultured P388/S cells. The degree of cell death in P388/DOX cells induced by hydrogen peroxide was less than that in P388/S cells treated with hydrogen peroxide. Parent cells exposed to 3 mM of hydrogen peroxide showed a loss of mitochondrial membrane potential correlated with cell death. Hydrogen peroxide at a concentration greater than 0.3 mM increased the intracellular Ca^2+ of P388/S cells dose-dependently ; however, no change following addition of hydrogen peroxide (0.3-1mM) was observed in the resistant cells. Hydrogen peroxide (0.1 and 1 mM) treatment also induced the production of intracellular ROS in P388/S cells, while no such increase was produced by this substance in P388/DOX cells. Resistant cells also showed a significant level of glutathione (GSH) compared with the parent cells. In addition, N-acetyl-L-cysteine and reduced GSH antioxidants abolished death of P388/S cells caused by hydrogen peroxide. Therefore, it is believed that the reduced effect of oxidative stress towards the resistant cells may be related to an increase in intracellular GSH level.

• 社団法人日本薬学会の論文
• 2001-05-01

著者

• Sakaguchi Shuhei

  東北薬科大学 第一衛生化学

• Sakaguchi Shuhei

  First Department Of Hygienic Chemistry Tohoku Pharmaceutical University

• Satoh S

  Department Of Pharmacology And Pharmacotherapy Nihon Pharmaceutical University

• Satoh Susumu

  Nagasaki Institute Of Applied Science

• TAKAYANAGI Motoaki

  Department of Pathophysiology, Tohoku Pharmaceutical University

• Sasaki K

  Dep. Of Pharmacology And Toxicology Cancer Res. Inst. Tohoku Pharmaceutical Univ.

• Takayanagi M

  Department Of Pathophysiology Tohoku Pharmaceutical University

• Takayanagi Motoaki

  Department Of Pharmacology And Toxicology Cancer Research Institute Tohoku Pharmaceutical University

• Takayanagi Motoaki

  Cancer Research Institute Tohoku Pharmaceutical University

• FURUSAWA Shinobu

  Department of pharmacology and Toxicology, Cancer Research Institute, Tohoku Pharmaceutical Universi

• SASAKI Ken-ichi

  Department of pharmacology and Toxicology, Cancer Research Institute, Tohoku Pharmaceutical Universi

• TAKAYANAGI Yoshio

  Department of Pharmacology and Toxicology, Cancer Research Institite, Tohoku Phamaceutical Universit

• KIMURA Etsuko

  Department of Pharmacology and Toxicology, Cancer Research Institute, Tohoku Pharmaceutical Universi

• KISARA Satoko

  Department of Pharmacology and Toxicology, Cancer Research Institute, Tohoku Pharmaceutical Universi

• NAKANO Shinya

  Department of Pharmacology and Toxicology, Cancer Research Institute, Tohoku Pharmaceutical Universi

• MURATA Ryo

  First Department of Pharmaceutics, Tohoku Pharmaceutical University

• TANAKA Yorihisa

  First Department of Pharmaceutics, Tohoku Pharmaceutical University

• FURUSAWA Shinobu

  Cancer Research Institute, Tohoku Pharmaceutical University

• Ishikawa Masaaki

  東北薬科大学癌研究所

• Tanaka Y

  Kyushu Univ. Fukuoka Jpn

• Kimura E

  Department Of Pharmacology And Toxicology Cancer Research Institute Tohoku Pharmaceutical University

• Takayanagi Y

  Department Of Pharmacology And Toxicology Cancer Research Institute Tohoku Pharmaceutical University

• Murata R

  First Department Of Pharmaceutics Tohoku Pharmaceutical University

• Kisara Satoko

  Department Of Pharmacology And Toxicology Cancer Research Institute Tohoku Pharmaceutical University

• Kanno Shu-ichi

  Department Of Pharmacology And Toxicology Cancer Research Institute Tohoku Pharmaceutical University

• SASAKI Kenichi

  Cancer Research Institute, Tohoku College of Pharmacy

• Tanaka Y

  Toho Univ. School Of Pharmaceutical Sci. Chiba Jpn

• Tanaka Yorihisa

  Department Of Drug Metabolism And Pharmacokinetics Tohoku Pharmaceutical University

• Sasaki Ken-ichi

  Department Of Pharmacology And Toxicology Cancer Research Institute Tohoku Pharmaceutical University

• Nakano S

  Department Of Pharmacology And Toxicology Cancer Research Institute Tohoku Pharmaceutical University

• Sasaki Ken-ichi

  Department Of Pathology Sapporo Medical University School Of Medicine

• Sasaki Ken-ichi

  Department Of Chemistry Faculty Of Science Hiroshima University

• Tanaka Yoshitaka

  Department of Drug Metabolism and Pharmacokinetics, Tohoku Pharmaceutical University

• Sakaguchi Shuhei

  First Department of Hygienic Chemistry ; Tohoku Pharmaceutical University

関連論文

• Effects of Selenium Deficiency on Aldehyde Oxidase 1 in Rats(Molecular and Cell Biology)
• Intrathecally Administered D-Cycloserine Produces Nociceptive Behavior Through the Activation of N-Methyl-D-aspartate Receptor Ion-Channel Complex Acting on the Glycine Recognition Site
• Anti-inflammatory Effect of Propolis through Inhibition of Nitric Oxide Production on Carrageenin-Induced Mouse Paw Edema(Pharmacology)
• Stress/Strain Controlling Parameter of Cracked Plate with Mechanical Heterogeneity
• Effect of Mechanical Heterogeneity on Fracture Toughness Evaluation of Steel Welds
• A Proposal of Generalized COD Criterion and Its Application to Welds
• Stresses-Strains and Fracture Behavior of 9% Ni Steel Weldments
• Crack Tip Plastic Deformation of Notched Plates with Mechanical Heterogeneity
• A Proposal on 3 Point Bending COD Test Method for Welded Joint of 9% Ni Steel
• Inhibition of Eosinophil Survival by a Selective Inhibitor of Phosphodiesterase 4 via the Induction of Apoptosis(Biochemistry/Molecular Biology)
• Trimidox-Induced Apoptosis Is Mediated Through Induction of p53 in NALM-6 Cells
• Inhibitory Effect of Propolis on the Growth of Human Leukemia U937 (Toxicology)
• Circumvention of Acquired Resistance to Doxorubicin in K562 Human Leukemia Cells by Oxatomide
• Reversal of Vinblastine Resistance in Human Leukemic Cells by Haloperidol and Dihydrohaloperidol
• Mechanism of Resistance to Oxidative Stress in Doxorubicin Resistant Cells
• Potentiation of Acetaminophen Hepatotoxicity by Doxapram in Mouse Primary Cultured Hepatocytes
• Reversal of Multidrug Resistance in Human Leukemia K562 by Tamolarizine, a Novel Calcium Antagonist
• Role of Endogenous Endotoxin on Tumor Necrosis Factor-Hypersensitivity Caused by D-Galactosamine Challenge
• Reversal of Acquired Resistance to Doxorubicin in K562 Human Leukemia Cells by Astemizole
• Characterization of Hydrogen Peroxide-Induced Apoptosis in Mouse Primary Cultured Hepatocytes
• Exposure to Hydrogen Peroxide Induces Cell Death via Apoptosis in Primary Cultured Mouse Hepatocytes
• Potentiation of Acetaminophen Hepatotoxicity and Mortality by Doxapram in Mice
• Thalidomide Promotes the Release of Tumor Necrosis Factor-α (TNF-α) and Lethality by Lipopolysaccharide in Mice
• Potentiation of Pirarubicin Activity in Multidrug Resistant Cells by Rifampicin
• Effects of Antitumor Activity and Protection of Shock Symptoms by a Traditional Chinese Medicine (Sho-Saiko-To) in Recombinant Human Tumor Necrosis Factor Administered Mice
• The Distinctive Effects of Acute and Chronic Psychological Stress on Airway Inflammation in a Murine Model of Allergic Asthma
• Separation of Caffeine from Supercritical Carbon Dioxide with a Zeolite Membrane
• Effect of Solute Adsorption Properties on Its Separation from Supercritical Carbon Dioxide with a Thin Porous Silica Membrane
• Inhibition of Hepatic Mixed-Function Oxidase Enzymes in Mice by Acute and Chronic Treatment with Selenium
• Inhibition of Hepatic Drug Biotransformation by Carrageenan-Induced Inflammation in the Rat : Effect of Sex Hormone Alterations
• Effect of Carrageenan-Induced Inflammation on the Induction of Hepatic Microsomal Enzymes by Phenobarbital and Benzo [α] pyrene in Male Rats
• Hepatic Drug Metabolizing Activity in Rats with Carrageenan-Induced Inflammation
• The Expression of mRNA for Calcitonin Gene-Related Peptide Receptors in a Mucosal Type Mast Cell Line, RBL-2H3(Biochemistry/Molecular Biology)
• Effect of Dietary cabbage Fermentation Extract and Young Barley Leaf Powder on Immune Function of Sprague-Dawley Rats
• High-Rate Deposition of Microcrystalline Silicon Photovoltaic Active Layers by Plasma-Enhanced Chemical Vapor Deposition at Kilo-Pascal Pressures
• Hot Water Extract of Bark of Nikko Maple (Acer nikoense) Induces Apoptosis in Leukemia Cells
• Apoptotic Cell Death Induced by Physarumin (Hemagglutinin from Myxomycete, Physarum polycephalum)
• Edge States of Zigzag Boron Nitride Nanoribbons
• Pseudospin and Deformation-Induced Gauge Field in Graphene(Interaction and Nanostructural Effects in Low-Dimensional Systems)
• Magnetism as a Mass Term of the Edge States in Graphene(Condensed matter : electronic structure and electrical, magnetic, and optical properties)
• Reduction of Cyclophosphamide-Induced Toxicity by Diethyldithiocarbamate and Carbon Disulfide and Its Possible Mechanism
• BIPHASIC EFFECT OF DOXAPRAM ON HYPNOTIC ACTIVITY OF PENTOBARBITAL IN MICE
• Lysosomal Cysteine Protease, Cathepsin H, Is Targeted to Lysosomes by the Mannose 6-Phosphate-Independent System in Rat Hepatocytes
• Lysosomal Cystein Protease, Cathepsin B, Is Targeted to Lysosomes by the Mannose 6-Phosphate-Independent Pathway in Rat Hepatocytes: Site-Specific Phosphorylation in Oligosaccharides of the Proregion
• Stereoselective Pharmacokinetics of RS-8359,A Selective and Reversible Inhibitor of A-Type Monoamine Oxidase, in Rats, Mice, Dogs, and Monkeys
• Delayed Release of Prostaglandins from Arachidonic Acid and Kinetic Changes in Prostaglandin H Synthase Activity on the Induction of Prostaglandin H Synthase-2 after Lipopolysaccharide-Treatment of RAW264.7 Macrophage-Like Cells
• Quantitative determination of diol metabolites of CS-670, a new antiinflammatory agent, by capillary column gas chromatography-mass spectrometry
• Functional Analysis of Aldehyde Oxidase Using Expressed Chimeric Enzyme between Monkey and Rat(Molecular and Cell Biology)
• Properties of 130 kDa Subunit of Monkey Aldehyde Oxidase(Molecular and Cell Biology)
• Cloning, Expression, and Characterization of Male Cynomolgus Monkey Liver Aldehyde Oxidase(Biochemistry)
• Substrate Selectivity of Monoamine Oxidase A, Monoamine Oxidase B, Diamine Oxidase, and Semicarbazide-Sensitive Amine Oxidase in COS-1 Expression Systems(Biochemistry)
• Molecular Cloning and Characterization of Rat Semicarbazide-Sensitive Amine Oxidase(Biochemistry/Molecular Biology)
• Trends in Antimicrobial Susceptibility of Streptococcus pneumoniae in the Tohoku District of Japan : A Longitudinal Analysis from 1998 to 2007
• Effect of Non-Insulin Dependent Diabetes on Cyclosporin A Disposition in Goto-Kakizaki (GK) Rats
• Effect of Streptozotocin-Induced Diabetes on Cyclosporin A Disposition in Rats
• Occludin modulates organization of perijunctional circumferential actin in rat endothelial cells
• Preventive Effects of a Traditional Chinese Medicine (Sho-saiko-to) on Septic Shock Symptoms ; Approached from Heme Metabolic Disorders in Endotoxemia(Pharmacognosy)
• Molecular and Sugar-Binding Heterogeneity of C-Type Lectins from Osmerus (Spirinchus) lanceolatus Eggs(Biochemistry/Molecular Biology)
• Catfish Egg Lectin Causes Rapid Activation of Multidrug Resistance 1 P-Glycoprotein as a Lipid Translocase(Biochemistry/Molecular Biology)
• DNA Aggregating Protein from Carp (Cyprinus capio) Eggs(Biochemistry/Molecular Biology)
• Phenotypic Conversion of SV-40-Immortalized Human Diploid Fibroblasts to Senescing Cells by Introduction of an Antisense Gene for SV40-T Antigen
• EFFECTS OF CHLORDIAZEPOXIDE, DIAZEPAM AND OXAZEPAM ON THE ANTITUMOR ACTIVITY, THE LETHALITY AND THE BLOOD LEVEL OF ACTIVE METABOLITES OF CYCLOPHOSPHAMIDE AND CYCLOPHOSPHAMIDE OXIDASE ACTIVITY IN MICE
• EFFECT OF DOXAPRAM ON THE ACTION OF OTHER DRUGS AND THE HEPATIC DRUG-METABOLIZING SYSTEM IN MICE
• DRUG INTERACTION OF ANTITUMOR DRUGS III. ANTITUMOR ACTIVITY OF TEGAFUR IN LIPOPOLYSACCHARIDE-TREATED MICE
• Preventive Effects of a Traditional Chinese Medicine(Sho-saiko-to)on Endotoxin-Induced Cytotoxicity and Tumor Necrosis Factor-α Production in J774A.1 Cells(Pharmacognosy)
• Synthesis of grafted polysilsesquioxane by ring-opening polymerization of lactide and trimethylene carbonate
• Chromatographic Resolution of a Diastereomeric Mixture of Tris(trans-1, 2-diaminocyclohexane)cobalt(III) by Bis(μ-tartrato)diantimonate(III)Anion and Association Modes Found in Their Diastereomeric Salts
• Theory of Superconductivity of Carbon Nanotubes and Graphene(Condensed matter: electronic structure and electrical, magnetic, and optical properties)
• Edge States of Zigzag Boron Nitride Nanoribbons
• Vacuum structure of π-electrons in carbon nanotubes(Abstracts of Doctoral Dissertations,Annual Report(from April 2002 to March 2003))
• Effect of sizofilan, an immunomodulator, on hepatic microsomal mixed-function oxidase activities in rats.
• The deleterious effect of buthionine sulfoximine, a glutathione-depleting agent, on the cisplatin toxicity in mice.
• Injurious effect of buthionine sulfoximine, an inhibitor of glutathione biosynthesis, on the lethality and urotoxicity of cyclophosphamide in mice.
• Exacerbation of Doxorubicin Toxicity by Chlorpromazine in Male ddY Mice.
• Endotoxin- and Inflammation-Induced Depression of the Hepatic Drug Metabolism in Rats.
• PATHOLOGICAL INVESTIGATIONS ON HEPATOCELLULAR CARCINOMA
• Isomers of the diamminebis(trimethylenediamine)cobalt(III) complex.

もっと見る 閉じる

スポンサーリンク