Inhibitory Effect of Propolis on the Growth of Human Leukemia U937 (Toxicology)
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概要
- 論文の詳細を見る
We have investigated the effect of propolis (CB Propolis) on the growth of human histiolytic lymphoma U937 cells. We found that propolis strongly inhibited the growth of the cells and macromolecular synthesis in a doseand time-dependent manner by apoptosis. Propolis at 0.015-0.5 μl/ml showed antitumor activity with an IC_<50> of 0.18 μl/ml for 3d. It also inhibits DNA, RNA and protein synthesis with an IC_<50> of 0.08, 0.17 and 4.3 μl/ml, respectively. The inhibitory effect on DNA synthesis was partially irreversible. Moreover, an apoptotic DNA ladder and chromatin condensation were observed in the same concentration range in which cell growth was inhibited. The caspase inhibitor, Z-Asp-CH_2-DCB, prevented DNA fragmentation. These results suggest that the antitumor activity of propolis occurs through the induction of apoptosis. Propolis may be useful as a cancer chemopreventive and chemotherapeutic agent.
- 社団法人日本薬学会の論文
- 2004-05-01
著者
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SATOH Susumu
Department of Pharmacology and Pharmacotherapy, Nihon Pharmaceutical University
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TADANO Takeshi
Department of Pharmacology, Tohoku Pharmaceutical University
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ISHIKAWA Masaaki
Department of Pharmacology and Toxicology, Cancer Research Institute, Tohoku Pharmaceutical Universi
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Tadano Takeshi
東北薬科大学 薬理学
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Tadano Takeshi
東北薬科大学 薬学部
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Tadano T
Tohoku Pharmaceutical Univ. Sendai Jpn
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Tadano T
Dep. Of Pharmacology Tohoku Pharmaceutical Univ.
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Tadano Takeshi
Department Of Pharmacology Tohoku Pharmaceutical University
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KANNO Syu-ichi
Department of Clinical Pharmacotherapeutics, Tohoku Pharmaceutical University
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Kanno Syu-ichi
Department Of Clinical Pharmacotherapeutics Tohoku Pharmaceutical University
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Kanno Syu-ichi
東北薬科大学癌研究所
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Tadano Takeshi
Department Of Pharmacology Tohoku College Of Pharmacy
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Satoh S
Department Of Pharmacology And Pharmacotherapy Nihon Pharmaceutical University
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Satoh Susumu
Nagasaki Institute Of Applied Science
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Kanno Syu-ichi
東北薬科大学 薬物治療学教室
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ASO Keiko
Department of Pharmacology and Toxicology, Cancer Research Institute, Tohoku Pharmaceutical Universi
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Aso Keiko
Department Of Endocrinology And Metabolism Tokyo Metropolitan Kiyose Children's Hospital
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Aso Keiko
Department Of Pharmacology And Toxicology Cancer Research Institute Tohoku Pharmaceutical University
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Kanno Syu-ichi
Department Of Pharmacology And Toxicology Cancer Research Institute Tohoku Pharmaceutical University
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Ishikawa Masaaki
Department Of Clinical Pharmacotherapeutics Tohoku Pharmaceutical University
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Ishikawa Masaaki
Department Of Pharmacology And Toxicology Cancer Research Institute Tohoku Pharmaceutical University
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Ishikawa Masaaki
Department Of Pharmacology And Toxicology Cancer Research Institute Tohoku College Of Pharmacy
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Satoh Susumu
Department Of Neurosurgery Yamagata Prefectural Central Hospital
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Ishikawa Masaaki
Dep. Of Clinical Pharmacotheraputics Tohoku Pharmaceutical Univ. Jpn
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TADANO Takeshi
Department of Chemical Pharmacology, Tohoku College of Pharmacy
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