Preventive Effects of a Traditional Chinese Medicine(Sho-saiko-to)on Endotoxin-Induced Cytotoxicity and Tumor Necrosis Factor-α Production in J774A.1 Cells(Pharmacognosy)

概要

論文の詳細を見る
Sho-saiko-to, one of the most frequently prescribed Kampo medicines, is used clinically to treat chronic hepatitis and has shown confirmed clinical efficacy. The present study investigated whether Sho-saiko-to can suppress cytotoxicity and tumor necrosis factor (TNF) -α production in endotoxin-treated J774A.1 cells. Sho-saiko-to (10-20μg/ml) did not affect the proliferation of J774A.1 cells, while a high concentration (50μg/ml) of Sho-saiko-to induced a slight reduction in cell viability. Treatment with Sho-saiko-to (10-50μg/ml) significantly inhibited endotoxin (10μg/ml) -induced cytotoxicity in J774A.1 cells. In addition, Sho-saiko-to (20μg/ml) suppressed TNF-α production by endotoxin (1μg/ml) -activated J774A.1 cells. These findings suggest that the Kampo prescription Sho-saiko-to suppresses cytotoxicity or TNF-α production in macrophages treated with endotoxin and that it may be useful in improving septic shock symptoms. Sho-saiko-to may therefore protect against some of the various disturbances caused by endotoxins through its ability to inhibit TNF-α production in septic shock.
公益社団法人日本薬学会の論文
2004-09-01