Structure-Activity Relationships of Lipopolysaccharide (LPS) in Tumor Necrosis Factor-α (TNF-α) Production and Induction of Macrophage Cell Death in the Presence of Cycloheximide (CHX) in a Murine MacrophageLike Cell Line, J774.1
スポンサーリンク
概要
- 論文の詳細を見る
The structure-activity relationships of lipopolysaccharide (LPS) in tumor necrosis factor-α (TNF-α) production and induction of macrophage cell death in the presence of cycloheximide (CHX) were examined in a murine macrophage-like cell line, J774.1. TNF-α production is one of the characteristic phenotypes of LPS-activated macrophages, and is observed upon incubation with LPS. On the contrary, macrophage cell death, which had been found in our laboratory (Amano F., Karahashi H., J. Endotoxin Res., 3,415-423 (1996)) and was examined as the release of lactate dehydrogenase (LDH) from cells into the culture supernatant, was observed 2.5 h after the addition of LPS in the presence of CHX. However, both TNF-α production and macrophage cell death were similarly dependent on the structures of LPS and lipid A. At more than 10 ng/ml, wild-type LPS from E.coli and S. minnesota exhibited the highest activity, and LPS as well as diphosphoryl lipid A from S. minnesota rough mutants also exhibited activity, but E. coli LPS detoxified by alkaline treatment or monophosphoryl lipid A from S. minnesota exhibited no activity even at 100 ng/ml. These results suggest that LPS-induced macrophage cell death in the presence of CHX shows similar requirements for LPS and/or lipid A structures as for the macrophage activation at higher doses than 10 ng/ml, although the former was not observed at 1 ng/ml LPS, while the latter was. However, TNF-α does not seem to be involved in the induction of macrophage cell death, because a neutralizing anti-TNF-α antibody failed to block the macrophage cell death and because recombinant TNF-α had little effect on the extent of LDH release in the presence or absence of LPS and/or CHX, and also because TNF-αproduction by LPS was abolished in the presence of CHX.
- 公益社団法人日本薬学会の論文
- 1998-10-15
著者
-
Amano Fumio
Department Of Biochemistry And Cell Biology National Institute Of Infectious Diseases
-
Amano Fumio
Department Of Biochemistry And Cell Biology National Institute Of Infectious Disease
-
Karahashi Hisae
Department Of Biochemistry And Cell Biology National Institute Of Infectious Diseases
-
Amano Fumio
Department Of Biochemistry And Cell Biology National Institute Of Health
関連論文
- Inhibitory Effects of Hydrolyzable Tannins form Melastoma dodecandrum LOUR. on Nitric Oxide Production by a Murine Macrophage-Like Cell Line, RAW264.7,Activated with Lipopolysaccharide and Interferon-γ
- Regulatory Role of C-Terminal Residues of SUlA in Its Degradation by Lon Protease in Escherichia coli
- Inhibition of Listeriolysin O-Induced Hemolysis by Bovine Lactoferrin
- Delayed Release of Prostaglandins from Arachidonic Acid and Kinetic Changes in Prostaglandin H Synthase Activity on the Induction of Prostaglandin H Synthase-2 after Lipopolysaccharide-Treatment of RAW264.7 Macrophage-Like Cells
- Defect in the Induction of Nitric Oxide Synthase by Lipopolysaccharide (LPS) in an LPS-Resistant Mutant of a Murine Macrophage-Like Cell Line, J774.1
- Endotoxin-Tolerance to the Cytotoxicity toward a Macrophage-Like Cell Line, J774.1, Induced by Lipopolysaccharide and Cycloheximide: Role of p38 MAPK in Induction of the Cytotoxicity(Highlighted paper selected by Editor-in-chief)(Biochemistry/Molecular Bi
- Changes of Caspase Activities Involved in Apoptosis of a Macrophage-Like Cell Line J774.1/JA-4 Treated with Lipopolysaccharide (LPS) and Cycloheximide
- Suppression of Prostaglandin Synthesis by Arachidonic Acid or Eicosapentaenoic Acid in a Macrophage-Like Cell Line, RAW 264.7,Treated with LPS
- Effects of Carrageenans on the Binding, Phagocytotic, and Killing Abilities of Macrophages to Salmonella(Food & Nutrition Science)
- Enhanced Release of Prostaglandin D_2 during Re-incubation of RAW 264.7 Macrophage-Like Cells after Treatment of Both Lipopolysaccharide and Non-steroidal Anti-inflammatory Drugs(Biochemistry/Molecular Biology)
- Effects of Anaerobic Preculture on Aerobic Stress Responses of Campylobacter jejuni
- Structure-Activity Relationships of Lipopolysaccharide (LPS) in Tumor Necrosis Factor-α (TNF-α) Production and Induction of Macrophage Cell Death in the Presence of Cycloheximide (CHX) in a Murine MacrophageLike Cell Line, J774.1
- Differences in Netric Oxide Synthase Activity in a Macrophase-Like Cell Line, RAW264.7 Cells, Treated with Lipopolysaccharide (LPS) in the Presence or Absence of Interferon-γ (IFN-γ): Possible Heterogeneity of iNOS Activity
- REVERSAL BY IFN_γ OF THE DEFECT IN LPS-INDUCED O_2-GENERATING ACTIVITY IN AN LPS-RESISTANT MUTANT, LPS1916 CELLS, DERIVED FROM A MACROPHAGELIKE CELL LINE, J774.1 CELLS