DNA-DNA交雑による火落菌の簡易同定法

概要

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Hiochi-bacteria, lactobacilli which grow and spoil sake, were identified by DNA-DNA hybridization. Digoxigenin-labelled total DNA probes and an ELISA detection kit were used for dot-blot hybridization. No significant homology was detected among 12 type strains of the genus Lactobacillus except for a trace signal among L. casei, L. paracasei, and L. rhamnosus. It was ascertained that L. homohiochii and L. heterohiochii were unique species of true hiochi-bacteria. Homo-fermentative hiochi-bacteria isolated by the present authors were identified as L. paracasei subsp. paracasei (12 isolates), and L. plantarum (2 isolates) ; none of them were of true hiochi-bacteria (L. homohiochii). Five strains of homo-fermentative hiochi-lactobacilli, which are stock cultures of the National Research Institute of Brewing (RIB), were identified as L. paracasei subsp. paracasei ; four strains of hetero-fermentative hiochi-lactobacilli isolated by the present authors were identified as L. hilgardii ; and four strains of hetero-fermentative hiochi-lactobacilli, which are also stock cultures of the RIB, were all identified as L. hilgardii.
社団法人日本生物工学会の論文
1995-01-25