アルカリo-フェニレンジアミン法におけるBacillus subtilis FT-3の生産する多糖の挙動 : Bacillus subtilis FT-3の生産する多糖に関する研究 (第5報)

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To evaluate the alkaline o-phenylenediamine (OPD) method of investigation of the chemical structure of polysaccharides, the method was applied to two polysaccharides produced by microorganisms, PLS and FT-3 polysaccharides, both of whose structures had already been determined.1. For both PLS and FT-3,the formation of quinoxalines was observed to increase steadily with time, suggesting they are predominantly as linear polymers.2. The ion-exchange chromatogram of the quinoxalines produced from PLS indicated sufficiently the component sugars and their linkages, as expected. However, the behavior of FT-3 was more complicated, suggesting that the consecutive quinoxaline formation from the main linear chain was accompanied by continous production of quinoxalines from free monosaccharides, reflecting the specificity in quinoxaline formation from 1,3-linked glucose residues having a galactosyl side chain in the C-4 position.3. PLS and FT-3 showed formation of similar acidic quinoxalines from galacturonic acid and glucuronic acid, respectively.4. Thin-layer chromatography of quinoxalines formed from both polysaccharides gave satisfactory separation of the quinoxalines and suggested the suitability of TLC of this kind for estimation of the structure of heteropolysaccharides.5. The mechanism of alkaline decomposition and quinoxaline formation of FT-3 under the conditions for the alkaline OPD method is discussed.
社団法人日本生物工学会の論文
1975-03-25

アルカリo-フェニレンジアミン法におけるBacillus subtilis FT-3の生産する多糖の挙動 : Bacillus subtilis FT-3の生産する多糖に関する研究 (第5報)

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