Bacillus subtilis FT-3の生産する多糖の部分構造について

概要

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Structural studies of FT-3 polysaccharide were carried out by partical acid hydrolysis, partial acetolysis, and Smith degradation. Partial acid hydrolysis of the polysaccharide readily liberated balactose as monosaccharide, but produced no disaccharide containing galactose. Partical acetolysis gave glucose, fucose, glucuronic acid, galactosyl-glucose and glucosylfucose togther with di-, tri- and tetrasaccharides containing glucuronic acid. Periodate oxidation of the polysaccharde showed that 0.59 mole of periodate was consumed and 0.25 mole of formic acid was formed per sugar residue. From Smith degradation of the polysaccharide, it was assumued that the galactose residue was situated at the non-reducing end, the glucuronic acid residue was linked through C_1 and C_4 and one of the glucose residues was linked through C_1 and C_4. The remainder of the glucoase residues and all fuose residues were found to be resistant to periodate oxidation. Oligosaccharides obtained from polysaccharide by Smith degradation were suggested to be fucosylglycosylerythronic acid, glucosylerythronic acid and fucosylglucosylerythritol, respectively. Consequently, Fuc-Glc-Glc-UA, Glc-GlcUA and Fuc-Glc-Glc were considered to form the main building units of the polysaccharide.
社団法人日本生物工学会の論文
1974-09-25