Bacillus subtilis FT-3の生産する多糖のメチル化法による構造の検討 : Bacillus subtilis FT-3の生産する多糖に関する研究 (第4報)

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The structure of FT-3 polysaccharide was studies by periodate oxidation and methylation.Permethylated FT-3 polysaccharide and permethylated polysaccharide polyalcohol 1 were hydrolyzed, reduced and subjected to acetylation to obtain the corresponding partially methylated alditol acetates. On the other hand, permethylated polysaccharide polyalcohol 1 was methanolyzed and subjected to trimethylsilylation.From gas chromatography and gas chromatography-mass spectrometry of the derivatives obtained above, it has been proved that the fucose residue was 1,3-linked (about 1.4 mole) and the glucuronic acid resi ue was 1,4-linked (1.0 mole), whereas glucose redidue was partly 1,4-linked (about 0.15 mole), and partly 1,3-linked (about 1.6 mole), the latter glucose residue involving one galactose residue at its C_4 position. From the above results together with previous knowledge obtained by the authors, the sequence of the basic unit of FT-3 polysaccharide is suggested to be[figure]
社団法人日本生物工学会の論文
1975-01-25