高濃度稲ワラの並行複発酵によるエタノールへの転換

概要

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When various concentrations of alkali-treated rice straw were hydrolyzed by a mixture of cellulases Acucelase from Aspergillus aculeatus and Meicelase from Trichoderma viride, 5-10% rice straw was well hydrolyzed. On the contrary 20-25% rice straw was hardly hydrolyzed, because of difficulties of stirring and product inhibition. To solve this barrier, simultaneous saccharification and fermentation of rice straw was examined. When 25% rice straw was fermented by a mixed cellulase, xylose isomerase and Saccharomycces fermentati IAM 4771,25% rice straw ws hydrolyzed almost completely, but ethanol yield was relatively low. This phenomenon seemed to be due to tolerance of the yeast to a high concentration of ethanol and β-1,3-glucanase contaminating the cellulase preparation. From screening for a suitable strain for simultaneous saccharification and fermentation, Saccharomyces cerevisiae AJ 4005 were selected. When 25% rice straw was fermented by a mixed cellulase, xylose isomerase and Saccharomyces cerevisiae AJ 4005,11.1-11.5% of ethanol was obtained. The cellulase concentration required was 0.5%. The ethanol yield was 92-95% of the theoretical value.
社団法人日本生物工学会の論文
1985-09-25