Aspergillus aculeatusとTrichoderma virideの混合セルラーゼによるセルロース性物質の糖化とアルコール発酵

概要

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Enzymatic hydrolysis of cellulosic substances was investigated with a view to fuel production from biomass. Ten % of rice straw, pretreated with 1% NaOH at 100℃ for 3 h, was hydrolyzed almost completely to monosaccharides by a mixture of Aspergillus aculeatus cellulase (Acucelase) and Trichoderma viride cellulase (Meicelase), and gave 9.5% sugar solution. Optimal conditions for enzymatic hydrolysis were as follows : pH 4.5,temperature 45℃, enzyme concentration 0.5% (as protein). Bagasse was also hydrolyzed in the same way as rice straw. Enzymatic hydrolyzates of rice straw and bagasse were converted to ethanol by Saccharomyces fermentati in the presence of glucose isomerase, which isomerizes xylose to xylulose, and gave 4.2% ethanol. The ethanol yield from sugar was 80-88% of the theoretical value.
社団法人日本生物工学会の論文
1983-11-25