羊毛クチクル分解酵素生産菌Bacillus cereus NS-11株の分離と分離菌の生産する酵素の性質

概要

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A wool cuticle-degrading bacterium was isolated from soil and identified as Bacillus cereus(strain NS-11). The cuticle-degrading enzyme produced by the bacterium had high activity against wool keratin rather than casein. The optimum pH of the enzyme was 8,and it was stable between pH 5 and 9. The enzyme easily hydrolyzed denatured keratin, such as that which was chemically modified, but hardly hydrolyzed native keratin. Amorphous wool keratin was hydrolyzed by the enzyme more quickly with the assistance of hydrogen peroxide, whereas hydrogen peroxide was of little assistance in the hydrolysis of pulverized wool fibers. However, in the presence of hydrogen peroxide the enzyme modified the cuticle components preferentially without damaging the inner components of the wool fibers. The enzyme produced by the isolated strain is thus expected to be effectual in the shrink proofing of wool without decreasing its tensile-strength or abrasion-resistance.
社団法人日本生物工学会の論文
1998-02-25