Bacillus Subtilis FT-3により生産される多糖の化学的及び物理化学的性質 : Bacillus Subtilis FT-3の生産する多糖に関する研究 (第2報)

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Some chemical and physicochemical properties of polysaccharde produced by Bacillus subtilis FT-3 were investigated.1. FT-3 polysaccharide was an O-acetylated polysaccharide, the components of which are glucose, galactose, fucose, glucuronic acid and acertl in the molar ratio of 2 : 2 : 1 : 1 : 1.4.2. Neutralization equivalent, intrinsic visocoseity, _s ^o _20,w and partial specific volume of FT-3 polysaccgaride were 953,4.8 (g/100 ml)^<-1>, 2.52 S and 0.64 ml/g, respectively.3. The length and width of the molecule of FT-3 polysaccharid reduced from birefrigent measurements were about 2100 A and 19.3 A.4. The mean molecular weights were estimated to be 15.6×10^4,100〜200×10^4,3.5×10^4<DP^^^-_n=200>and 2.5×10^4<DP^^^--n=150>based on the resilts by Scheraga Mandelkern' equation, gel filtration on Sepharose 4 B, Somogyi Nelson's method and determination of HCHO liberated by oxidation of the borohydride-reduced polysaccharide, respectively.5. this polysaccharide appeared to have an O-acyl group and a β-glycosidic linkage in the molecule based on the results of infrated analysis.6. β-galactosidase from Charonia lampas liberated 2.5% of galactose from the non-reducing terminal or side-chain of FT-3 oplysaccharide.
社団法人日本生物工学会の論文
1974-07-25