アミラーゼ阻害剤, S-AI添加によるBacillus polymyxaのβ-アミラーゼの増産

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Enhancement of β-amylase production of Bacillus polymyxa by S-AI, an amylase inhibitor. OHAYAMA K. and S. MURAO (Department of Agricultural chemistry, College of Agriculture, University of Osaka Prefecture, Sakai, Osaka 591) Hokkokogaku 57 : 1-5. 1979. S-AI is a potent α-amylase inhibitor produced by Streptomyces diastaticus subsp. amylostaticus no. 2476. It is though to be an oligosaccharide composed of 6 molecules of glucose and 1 molecule of S-AI-X (unknown structure). A small amount of S-AI (5-10 μg/ml) in the medium enhanced the β-amylase productrion of Bacillus polymyxa no. 72 by about 2-to 3-fold. Addition of S-AI at any time before maximum growth was reached was most effective and thereafter the effeft gradually decreased. This effect of S-AI depended on the concentration of soluble starch in the medium. The whle of S-AI was not involved in the effect, because β-amylase hydrolyzate of S-AI (glucose-S-AIX-glucose) showed the same effect as native S-AI. β-Amylase was purified from the filtrate of the culture with added S-AI and its properties were compared with those of the enzyme obtained from the control culture without S-AI. No difference was found between the two.
社団法人日本生物工学会の論文
1979-01-25