Basidiomycetes の深部培養に関する研究 : (第2報) 椎茸菌 (Lentinus edodes) の呼吸におよぼす酸素の影響について

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It was previously reported that the growth rate of Shiitake (Lentinus edodes) in submerged culture was effected by the coefficient of the oxygen absorption (K_d) of the medium. Another most important problem is the action of cells on oxygen molecules, which is described here. The effect of dissolved and gaseous oxygen concentrations on the respiration rate of Shiitake mycelium in both surface and submerged cultures were investigated.1. Affinity between Shiitake mycelia and oxygen Mycelia of Submerged culture had a Michaelis-Menten type of action against both dissolved and gaseous oxygen, and two affinity index pK_m appeared with the growth of mycelia pellets, which may be attributed to the resitsance against diffusion of oxygen within the pellet. Mycelia of surfae culture had a Michaelis-Menten type action against gaseous oxygen and the pK_m' value was 2.4,which was higher than the value of mycelia of submerged culture, but mycelia of surface culture had a monomolecular reaction against dissolved oxygen.2.Change of affinity index correlating with the change of oxygen supply condition According to the change of oxygen supply condition, pK_m' values easily changed.Although it was not easy for mycelia of surface culture to be adapted to dissolved oxygen, the replacement of mycelia to the new medium was effective for the increasing of the pK_m value.
社団法人日本生物工学会の論文
1965-12-25