Influence of Medium Components and Growth Conditions on Pediocin Iz3.13 Production by Pediococcus pentosaceus Iz3.13, Isolated from Japanese Traditional Fermented Seafood
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概要
- 論文の詳細を見る
Growth requirements for maximum production of pediocin Iz3.13 by Pediococcus pentosaceus Iz3.13 were studied. Physical and chemical parameters that enhanced pediocin production included incubation in static broth at 30℃, with an initial pH of 4.5. Optimum nutritional conditions that enhanced pediocin Iz3.13 production were 3.5% Trypticase peptone, 3.5% Phytone peptone or 5.0% Proteose peptone No.3 as nitrogen sources, and glucose or maltose as carbon sources. Supplementing growth media with beef extract and MgCl2 also enhanced production. This study proposes an optimized growth medium containing 5.0% Proteose peptone No.3, 3.5% Phytone peptone, 2.5% glucose, 1.5% MgCl2, 3.0% beef extract and 0.25% K2HPO4. This medium significantly enhanced pediocin Iz3.13 production (5.1 fold increase) as compared to a basal medium.
- 2010-03-09
著者
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Bagenda Dominic
Department Of Media Architecture Future University-hakodate
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川合 祐史
北海道大学大学院水産科学研究院
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川合 祐史
北大院水産
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KOBAYASHI Tetsuya
Faculty of Fisheries Sciences, Hokkaido University
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Kobayashi Tetsuya
Faculty Of Fisheries Sciences Hokkaido University
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Kawai Yuji
Faculty Of Fisheries Sciences Hokkaido University
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Kawai Yuji
Division Of Marine Life Science Faculty Of Fisheries Sciences Hokkaido University
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Kawai Yuji
Laboratory Of Marine Food Technology Faculty Of Fisheries Hokkaido University
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川合 祐史
北海道大学水産学部
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Kawai Y
Faculty Of Fisheries Sciences Hokkaido University
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