Purification and Characterization of NADPH-dependent 2-Oxoaldehyde Reductase from Chicken Liver(Biological Chemistry)
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概要
- 論文の詳細を見る
The enzymes which metabolize 2-oxoaldehyde compounds in a crude extract of chicken liver were examined using 3-deoxyglucosone (3-DG) and methylglyoxal (MG). NADH- and NADPH-dependent reductase activities were observed and the latter was more than the former. NADPH-dependent 2-oxoaldehyde reductase was purified from chicken liver by ammonium sulfate fractionation, and DEAE-cellulose, CM-cellulose, hydroxylapatite, and Sephadex G-100 column chromatographies. The molecular weight of the enzyme was estimated to be 38,000 and 32,000 by SDS-PAGE and gel filtration, respectively. The optimum pH was 6.5-7.0 and this enzyme was stable at pH 7.0-8.0. The Km for 3-DG and MG were 3.75 mM and 1.52 mM, respectively. This enzyme had high activities towards 2-oxoaldehyde compounds, such as 3-DG, MG, and phenylglyoxal. This enzyme converted 3-deoxyglucosone (3-DG) to 3-deoxyfructose, and methylglyoxal (MG) to acetol.
- 社団法人日本農芸化学会の論文
- 1991-04-23
著者
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早瀬 文孝
明治大学農学部農芸化学科
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Hayase Fumitaka
Department of Agricultural Chemistry, Meiji University
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Kato Hiromichi
Department of Agricultural Chemistry, The University of Tokyo
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Kato H
Univ. Tokyo Tokyo Jpn
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Kato H
Department Of' Agricultural Chemistry The University Of Tokyo
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Kato Hiromichi
Department Of Agricultural Chemistry The University Of Tokyo
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Kato Hiromichi
Department Of Food Science Ohtsuma University
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Kato H
Otsuma Women's Univ. Tokyo Jpn
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Hayase Fumitaka
Department Of Agricultural Chemistry Faculty Of Agriculture Meiji University
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Hayase Fumitaka
Department Of Agricultural Chemistry The University Of Tokyo:(present Address) Department Of Agricul
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Nishimura Toshihide
Department Of' Agricultural Chemistry The University Of Tokyo
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Nishimura Toshihide
Department Of Agricultural Chemistry The University Of Tokyo
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Hayase F
Department Of Agricultural Chemistry Meiji University
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Shin Hae
Department Of Agricultural Chemistry The University Of Tokyo
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