Studies of Nucleosides and Nucleotides. XXXVII. Synthesis of 8-Oxyguanosine Nucleotides and Uric Acid 9-Riboside 5'-Phosphate
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概要
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8-Oxyguanosine 5'-mono-, 5'-di- and 5'-tri-phosphate were synthesized from 2', 3'-O-isopropylidene-8-oxyguanosine by the phosphorylation with P^1-diphenyl, P^2-morpholinopyrophosphoro chloridate, follwed either by acidic removal of the protecting group, reaction with inorganic phosphate in the presence of DCC, or by the reaction of its morpholidate with pyrophosphate. 5'-Monophosphate was hydrolyzed by the catalysis with snake venom 5'-nucleotidase. 8-Oxyguanosine 2', 3'-cyclic phosphate, which was obtained by the direct phosphorylation method from 5'-O-acetyl-8-oxyguanosine, was completely resistant against pancreatic RNase and RNase T_1. Deamination of 8-oxyguanosine and its monophosphate was investigated. In the presence of excess nitrous acid a rapid loss of ultraviolet absorption was observed. With the use of limited amount of nitrous acid, uric acid 9-riboside 5'-monophosphate was obtained.
- 1968-07-25
著者
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池原 森男
Faculty Of Pharmaceutical Sciences Osaka University
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村尾 捷利
Faculty of Pharmaceutical Sciences, Hokkaido University
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村尾 捷利
Faculty Of Pharmaceutical Sciences Hokkaido University
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