Chemical Synthesis of Deoxyribonucleotide with a 5'-Phosphoryl Group on a Polystyrene Polymer Support by the Phosphotriester Method(Organic,Chemical)
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概要
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Highly lipophilic amines [trityloxyethylamine(TEAm) and N-trityloxyethylaniline(TEAn)] were introduced onto a 5'-phosphoryl group and the stability of the phosphoramidate linkage to acid was studied at the diester level. It was found that the phosphoramidate linkage using TEAn was cleaved by 80% aqueous acetic acid within 1 h at room temperature. However, it required 2 h to remove TEAm from the phosphate. By using the TEAn group, 5'-phosphorylated pentadecadeoxyribonucleotide pTCCAGGGTCTGGTAC was synthesized on a polystyrene support by the phosphotriester method. After partial deblocking, the pentadecamer with the TEAn group could be easily isolated on a reversed-phase column due to the high lipophilicity of the TEAn group. The chemically synthesized 5'-phosphorylated pentadecamer was successfully joined by using deoxyribonucleic acid ligase in the presence of a template.
- 公益社団法人日本薬学会の論文
- 1987-07-25
著者
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田中 俊樹
Faculty Of Pharmaceutical Sciences Osaka University:(present Address)protein Engineering Research In
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池原 森男
Faculty of Pharmaceutical Sciences, Osaka University(Present address)
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山田 泰樹
Faculty of Pharmaceutical Sciences, Osaka University
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山田 泰樹
Faculty Of Pharmaceutical Sciences Osaka University
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池原 森男
Faculty Of Pharmaceutical Sciences Osaka University
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