Synthesis and Properties of d(ATACGCGTAT) and Its Derivatives Containing One and Two 5-Methylcytosine Residues. : Effect of the Methylation on Deoxyribonucleic Acid Conformation
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概要
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In order to elucidate the effects of cytosine methylation on deoxyribonucleic acid (DNA) conformation, three self-complementary deoxyribodecanucleotides, d(ATACGCGTAT), d(ATACGm^5CGTAT) and d(ATAm^5CGm^5 CGTAT), were synthesized by a modified phosphotriester method and their conformational properties were examined by ultraviolet (UV) and circular dichroism (CD) spectroscopy. The two modified decamers were completely resistant to restriction endonuclease MluI, whose recognition sequence is ACGCGT, while the unmodified decamer was completely cleaved by the enzyme. All the decamers were assumed to exist as a B-form duplex in 0.1 M NaCl. The UV-temperature profiles suggested that the modified decamer duplexes have slightly higher stability to thermal perturbation but their melting processes are significantly less cooperative, as compared to the case of the unmodified decamer. All three decamers showed similar CD spectra under various salt conditions, including 4M NaCl and 6M CsF. However, substantial differences in CD spectra were observed in 8M NaClO_4 at -20℃. Both modified decamers were assumed to exist as a Z-form duplex under these extreme conditions, and they showed similar UV and CD-temperature profiles which were quite different from those of the unmodified decamer.
- 社団法人日本薬学会の論文
- 1986-01-25
著者
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上杉 晴一
横浜国立大学工学部
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上杉 晴一
Faculty Of Engineering Yokohama National University
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池原 森男
Faculty of Pharmaceutical Sciences, Osaka University(Present address)
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冨田 研一
Faculty of Pharmaceutical Sciences, Osaka University
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宮代 博継
Faculty of Pharmaceutical Sciences, Osaka University
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冨田 研一
Faculty Of Pharmaceutical Sciences Osaka University
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Tomita K
Faculty Of Pharmaceutical Sciences Osaka University
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上杉 精一
Faculty Of Pharmaceutical Sciences Osaka University
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池原 森男
Faculty Of Pharmaceutical Sciences Osaka University
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宮代 博継
Faculty Of Pharmaceutical Sciences Osaka University
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上杉 晴一
Faculty Of Pharmaceutical Sciences Osaka University
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