A Large-Scale Synthesis of a Nonadecadeoxyribonucleotide Duplex Having a Sequence Identical to That of Phage φ80 O_R2 by the Phosphoro-p-anisidate Method
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概要
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A nonadecanucleotide duplex, dCATCACCATAATGTTCATT・dAATGAACATTATGGTGATG, having a sequence identical to that of phage φ80 O_R2 has been synthesized by the phosphoro-p-anisidate method. 5'-O-Dimethoxytrityl-N-protected deoxynucleotide 3'-(o-chlorophenyl)phosphates and N-protected deoxynucleoside 3'-(o-chlorophenyl)phosphoro-p-anisidates were used to prepare di- and trinucleotide blocks. Oligomers were elongated either in the 5'-direction by dedimethoxytritylation or in the 3'-direction by treatment with isoamyl nitrite, and isolated by chromatography on silica gel or alkylated silica gel. The deblocked nonadecamers were purified by reversed phase and ion-exchange chromatography and the duplex was isolated by gel filtration.
- 社団法人日本薬学会の論文
- 1986-09-25
著者
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池原 森男
Faculty of Pharmaceutical Sciences, Osaka University(Present address)
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大塚 栄子
Faculty of Pharmaceutical Sciences, Osaka University
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大塚 栄子
Faculty Of Pharmaceutical Sciences Hokkaido University
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白石 昌彦
Faculty of Pharmaceutical Sciences, Osaka University
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池原 森男
Faculty Of Pharmaceutical Sciences Osaka University
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白石 昌彦
Faculty Of Pharmaceutical Sciences Osaka University
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