Studies on Transfer Ribonucleic Acids and Related Compounds. X. Synthesis of the Yeast Tyrosine tRNA 5'-Terminal Oligonucleotides
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概要
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The pentanucleotide which has the sequence of 8th to 12th base of the 5'-terminus of yeast tyrosine transfer ribonucleic acid (tRNA), UpApm^2GpCpC (1) has been synthesized by stepwise addition of properly protected mononucleotides using dicyolohexylcarbodiimide. 5'-O-Monomethoxytrityl-N^2-methylguanosine 3'-phosphate was prepared by phosphorylation of the 5'-protected nucleoside to give the 2', 3'-cyclic phosphate and successive hydrolysis with ribonuclease (RNase) St. The isolated yields of the protected ditri, tetra-, and penta-nucleotides were 75,38,31,and 21%, respectively. The 5'-terminal tetranucleotide CpUpCpUp (3) corresponding to 1st to 4th nucleotides, was synthesized by condensation of the dinucleotide, Bz-C^<Bz> (OBz)-pU (OBz)-p (14) and the anilidate, C^<Bz> (OBz)-pU-(OBz)-pNHPh (16b) in a yield of 22%. The trinucleotide CpGpGp (2) spanning 5th to 7th nucleotides from the 5'-end was also prepared by the amidate method from the dinucleotide, MMTrC^<Bz> (OBz)-pG^<Bu> (OiBu)-p(18) and G^<1Bu> (OiBu)-pNHPhOCH_3 (19b) in a yield of 17%.
- 公益社団法人日本薬学会の論文
- 1976-04-25
著者
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池原 森男
Faculty of Pharmaceutical Sciences, Osaka University(Present address)
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大塚 栄子
Faculty Of Pharmaceutical Sciences Hokkaido University
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藤山 和男
Faculty Of Pharmaceutical Sciences Osaka University
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大橋 稔
Faculty of Pharmaceutical Sciences, Osaka University
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池原 森男
Faculty Of Pharmaceutical Sciences Osaka University
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大橋 稔
Faculty Of Pharmaceutical Sciences Osaka University
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