Studies on Transfer Ribonucleic Acids and Related Compounds. XXXI. Synthesis of the 3', 5'-Bisphosphorylated Hexanucleotide corresponding to Bases (72-77) of tRNA^<fMet> from E. coli and Its Base Transition Analog
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概要
- 論文の詳細を見る
A hexanucleotide pC-A-A-C-C-Ap, which corresponds to the 3'-end of tRNA^<fMet>, was synthesized as a bisphosphorylated form, which should be a suitable donor substrate for joining with RNA ligase. 3'-Phosphorylated oligonucleotides were condensed by a triester method and the 5'-terminus of the protected hexanucleotide was phosphorylated with dianilidophosphorochloridate after demonomethoxytritylation. A site-directed base transition mutant sequence C-G-A-C-C-Ap was also synthesized by the triester method using suitably protected 3'-phosphorylated oligonucleotides.
- 公益社団法人日本薬学会の論文
- 1980-01-25
著者
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田中 俊樹
Faculty Of Pharmaceutical Sciences Osaka University:(present Address)protein Engineering Research In
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池原 森男
Faculty of Pharmaceutical Sciences, Osaka University(Present address)
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大塚 栄子
Faculty Of Pharmaceutical Sciences Hokkaido University
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池原 森男
Faculty Of Pharmaceutical Sciences Osaka University
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