Polynucleotides. XX. Synthesis of an AUG Analog containing 8,5'-O-Cycloadenosine
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概要
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8,5'-Anhydro-8-oxyadenosine was phosphorylated using cyanoethylphosphate and dicyclohexylcarbodiimide (DCC) in dimethylformamide-pyridine. Treatment of the products with a mixture of 50% aqueous pyridine-triethylamine and DEAE-cellulose chromatography gave 2', 3'-cyclic phosphate in a yield of 48%. 8,5'-Anhydro-8-oxyadenosine 3'-phosphate was obtained by RNase M digestion of the cyclic phosphate. After protection with benzoic anhydride in tetraethylammonium benzoate buffer, the 3'-phosphate was condensed with 2'-O-benzoyluridylyl-(3'→5')-N^2,2', 3'-O-triisobutyrylguanosine by the use of DCC. DEAE-cellulose, Sephadex G-25 and G-15 column chromatography gave 8,5'-anhydro-8-oxyadenylyl-(3'→5')-uridylyl-(3'→5')-guanosine. The trinucleotide was characterized by degradation to cyclo Ap, Up and G with RNase M.
- 公益社団法人日本薬学会の論文
- 1974-01-25
著者
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池原 森男
Faculty of Pharmaceutical Sciences, Osaka University(Present address)
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大塚 栄子
Faculty Of Pharmaceutical Sciences Hokkaido University
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名倉 猛夫
Faculty of Pharmaceutical Sciences, Osaka University
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名倉 猛夫
Faculty Of Pharmaceutical Sciences Osaka University
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池原 森男
Faculty Of Pharmaceutical Sciences Osaka University
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