Studies on Transfer Ribonucleic Acids and Related Compounds. XXXIV. Stepwise Diester or Partial Triester Synthesis of Penta-to Octanucleotides Corresponding to Residues 41-46,47-54,61-65 and 66-71 of tRNA^<Met>_f of E. coli
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概要
- 論文の詳細を見る
A hexanucleotide and an octanucleotide corresponding to tRNA^<Met>_f bases 41-46 and 47-54 were synthesized by stepwise addition of mononucleotides. The octanucleotide is the largest oligoribonucleotide synthesized by this method. A pentanucleotide (bases 61-65) and a hexanucleotide (bases 66-71) were synthesized via partially triesterified intermediates. The deblocked products were purified by ion-exchange chromatography and characterized by enzymatic hydrolysis. These oligonucleotides were used as substrates in joining reactions with RNA ligase.
- 社団法人日本薬学会の論文
- 1980-08-25
著者
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池原 森男
Faculty of Pharmaceutical Sciences, Osaka University(Present address)
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大塚 栄子
Faculty of Pharmaceutical Sciences, Osaka University
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大塚 栄子
Faculty Of Pharmaceutical Sciences Hokkaido University
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三宅 哲雄
Faculty of Pharmaceutical Sciences, Osaka University
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三宅 哲雄
Faculty Of Pharmaceutical Sciences Osaka University
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中川 栄子
Faculty of Pharmaceutical Sciences, Osaka University
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MARKHAM ALEXANDER
Faculty of Pharmaceutical Sciences, Osaka University
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MARKHAM ALEXANDERF.
Faculty of Pharmaceutical Sciences, Osaka University
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Markham Alexanderf.
Faculty Of Pharmaceutical Sciences Osaka University
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中川 栄子
Faculty Of Pharmaceutical Sciences Osaka University
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池原 森男
Faculty Of Pharmaceutical Sciences Osaka University
関連論文
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- Synthesis of 8,5'-Anhydro-8-mercaptoadenosine
- Synthesis of 8,5'-Anhydro-8-mercaptoadenosine
- Synthesis of 8,5'-Anhydro-8-mercaptoadenosine
- Studies on tRNA and Related Compounds. XXXVII. Synthesis and Physical Properties of 2'- or 3'-O-(o-Nitrobenzyl) nucleosides : the Use of o-Nitrophenyldiazomethane as a Synthetic Reagent
- Studies on Transfer Ribonucleic Acids and Related Compounds. XVI. Synthesis of Ribooligonucleotides using a Photosensitive o-Nitrobenzyl Protection for the 2'-Hydroxyl Group
- Studies on tRNA and Related Compounds. XXXVII. Synthesis and Physical Properties of 2'- or 3'-O-(o-Nitrobenzyl) nucleosides : the Use of o-Nitrophenyldiazomethane as a Synthetic Reagent
- Studies on Transfer Ribonucleic Acids and Related Compounds. XVI. Synthesis of Ribooligonucleotides using a Photosensitive o-Nitrobenzyl Protection for the 2'-Hydroxyl Group
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- Polynucleotides. LV. Synthesis and Properties of Dinucleoside Monophosphates derived from Adenine 8,2'-S- and Uracil 6,2'-O-Cyclonucleosides. Further Support for the Left-Handed Stacking of Oligonucleotides having High-Anti Base Torsion Angles
- Polynucleotides. LIII. Synthesis and Properties of 2'-Azido-2'-deoxyadenylyl-(3'-5')-2'-azido-2'-deoxyadenosine
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- Studies on Deoxyribonucleic Acids and Related Compounds. II. Synthesis of a Decanucleotide containing a Restriction Enzyme (PstI) Recognition Site
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- Studies on Transfer Ribonucleic Acids and Related Compounds. VII. Synthesis and Properties of Cyclic Oligoadenylic Acids
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- Debenzylation Reaction of Benzylphosphoric Acid Esters
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- Synthesis of Oligoribonucleotides via the Phosphite-Triester Approach on a Polymer Support
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- Polynucleotides. XXIX. Synthesis of Deoxyribooligonucleotide Blocks by an Extraction Method
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- Polynucleotides. I. Formation of Internucleotidic Linkage by Means of Dimethylformamide-Thionyl Chloride Complex
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