Activity of Artificial Mutant Variants of Human Growth Hormone Deficient in a Disulfide Bond between Cys53 and Cys165
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概要
- 論文の詳細を見る
In order to understand the role of Cys53 and Cys165 of human growth hormone (hGH) in receptor-binding and biological activity, artificial mutant variants of hGH were prepared in Escherichia coli by in vitro mutagenesis. Variants of hGH were constructed by replacement of Cys165 with Ala ([Ala165]hGH) or Ser ([Ser165]hGH), by replacement of Cys53 with Ala ([Ala53]hGH), by replacement of Cys53 and Cys165 with Ala ([Ala53,Ala165]hGH), or by replacement of Cys53 with Ala and Cys165 with Ser ([Ala53,Ser165]hGH). All of the variants constructed as well as reduced hGH exhibited less biological activity than that of intact hGH, and the decreases in biological activity were almost equal, as measured by a sensitive biological assay for growth hormone : adipose conversion assay using 3T3-F422A cells. These variants also showed less receptor-binding activity than that of intact hGH.These results suggest that it is possible neither the residue Cys53 nor Cys165 is directly involved in the receptor binding, and that the disulfide bridge between Cys53 and Cys165 in hGH may not always be crucial for the biological activity, though necessary to express full hGH activity.
- 社団法人日本薬学会の論文
- 1991-01-25
著者
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内田 恵理子
国立医薬品食品衛生研究所遺伝子細胞医薬部
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上杉 晴一
横浜国立大学工学部
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上杉 晴一
Faculty Of Engineering Yokohama National University
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田中 俊樹
Faculty Of Pharmaceutical Sciences Osaka University:(present Address)protein Engineering Research In
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森川 實
Tokyo Institute For Immunopharmacology Inc.
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内田 恵理子
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences
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池原 森男
Faculty of Pharmaceutical Sciences, Osaka University(Present address)
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田中 彰
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences(Present addr
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西川 諭
Faculty of Pharmaceutical Sciences, Osaka University
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早川 堯夫
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences
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上村 春樹
Faculty of Pharmaceutical Sciences, Osaka University
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西川 諭
産総研・生物機能工学
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早川 尭夫
国立医薬品食品衛生研究所
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内田 恵理子
国立衛研
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早川 堯夫
国立医薬品食品衛生研究所生物薬品部
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上村 春樹
Faculty Of Pharmaceutical Sciences Osaka University:(present Address)department Of Pathology New Yor
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Morikawa M
Tokyo Institute For Immunopharmacology Inc.
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Tanaka A
Faculty Of Pharmaceutical Sciences Tokyo University Of Science
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田中 彰
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences(present Addre
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早川 尭夫
Division Of Biological Chemistry And Reference Standards National Institute Of Hygienic Sciences
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Uchida Eriko
Human Genome Center Institute Of Medical Science University Of Tokyo
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上杉 精一
Faculty Of Pharmaceutical Sciences Osaka University
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池原 森男
Faculty Of Pharmaceutical Sciences Osaka University
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西川 諭
Faculty Of Pharmaceutical Sciences Osaka University
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上杉 晴一
Faculty Of Pharmaceutical Sciences Osaka University
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