Overproduction of Cellular and Activated Ha-ras Proteins by Mutating a Synthetic Gene(Biological,Chemical)
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概要
- 論文の詳細を見る
A synthetic gene for c-Ha-ras (Vat-12) has been modified by cassette mutagenesis using restriction sites, Cla1-BssHII, to encode c-Ha-ras Gly-12. Genes for c-Ha-ras (Leu-61 and Arg-61) have been synthesized by joining newly synthesized oligodeoxyribonucleotides containing appropriate codons, together with previously obtained synthetic fragments. These genes have been expressed in E. coli and the products (p21) isolated. The guanosine diphosphate binding properties and guanosine triphosphatase activities of these p21 derivatives were studied.
- 社団法人日本薬学会の論文
- 1987-12-25
著者
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池原 森男
Faculty of Pharmaceutical Sciences, Osaka University(Present address)
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大塚 栄子
北大・薬
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大塚 栄子
Faculty Of Pharmaceutical Sciences Hokkaido University
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大塚 栄子
北大
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紙谷 浩之
Faculty of Pharmaceutical Sciences, Hokkaido University
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三浦 一伸
Faculty of Pharmaceutical Sciences, Hokkaido University
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井上 泰彦
Faculty of Pharmaceutical Sciences, Hokkaido University
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井上 泰彦
Faculty Of Pharmaceutical Sciences Hokkaido University
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富永 峰子
Faculty of Pharmaceutical Sciences, Hokkaido University
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野口 茂
National Cancer Center Research Institute
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西村 進
National Cancer Center Research Institute
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紙谷 浩之
北大院・薬
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三浦 一伸
Faculty Of Pharmaceutical Sciences Hokkaido University
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富永 峰子
Faculty Of Pharmaceutical Sciences Hokkaido University
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池原 森男
Faculty Of Pharmaceutical Sciences Osaka University
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