Polynucleotides. LXIII. Solution Conformation of Oligodeoxyribonucleotides containing an Alternating dC-dG Sequence which can form a Left-handed Double Helix
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概要
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To elucidate the conformational properties and their chain length dependency in solution of oligodeoxyribonucleotides containing an alternating C-G sequence, which have been shown to take a left-handed duplex structure in crystals, d (C-G), d (C-G-C-G) and d (C-G-C-G-C-G) were synthesized by a modified triester method and characterized by ultraviolet (UV) circular dichroism (CD) and ^1H-nuclear magnetic resonance (NMR) spectroscopy. The effects of salt concentration (0.1 M and 4 M NaCl), oligomer strand concentration (10^<-5>-10^<-2>M) and temperature (0°-90℃) on the oligomer conformation were investigated. The dimer does not form a duplex under any conditions studied. The tetramer does form a right-handed duplex in 0.1M NaCl even at 3×10^<-5>M strand concentration. In 4M NaCl, however, it does not form any duplex at the same strand concentration, and left-handed duplex formation is observed at 10-fold higher concentration. The hexamer forms a duplex in either salt concentration even at 2×10^<-5>M strand concentration. The melting temperatures of these duplexes were measured by UV and CD methods. The CD-temperature profile in 4M NaCl, which is not sigmoidal when monitored at the λ_<min> of the characteristic negative band, suggests that melting of the high salt duplex is a rather complex process that involves more than two species. The results of ^1H-NMR-temperature studies support left-handed duplex formation in a high salt solution.
- 社団法人日本薬学会の論文
- 1981-12-25
著者
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上杉 晴一
横浜国立大学工学部
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上杉 晴一
Faculty Of Engineering Yokohama National University
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池原 森男
Faculty of Pharmaceutical Sciences, Osaka University(Present address)
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志田 敏夫
Faculty of Pharmaceutical Sciences, Osaka University
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志田 敏夫
Faculty Of Pharmaceutical Sciences Osaka University
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上杉 精一
Faculty Of Pharmaceutical Sciences Osaka University
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池原 森男
Faculty Of Pharmaceutical Sciences Osaka University
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上杉 晴一
Faculty Of Pharmaceutical Sciences Osaka University
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