Studies on Transfer Ribonucleic Acids and Related Compounds. XXI. : Synthesis and Properties of Guanine Rich Fragments from E. coli tRNA^<Met>_f 5'-End
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概要
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A hexanucleotide GpGpGpUpGpG corresponding to bases 5-10 of the tRNA^<Met>_f from E. coli. has been synthesized by stepwise addition of mononucleotides. The segment contained five guanine bases out of six nucleotides. We have improved the protection of the 2', 3'-hydroxyl groups of guanosine and guanosine 3'-phosphate by replacing to avoid undesirable deblocking during elongation of the chain. Condensation of 5'-O-monomethoxytrityl-2'-O-benzoyl-N-isobutyrylguanosine 3'-phosphate (9) with 2', 3'-O-dibenzoyl-N-isobutyrylguansoine (5) gave protected GpG in a yield of 62%. Subsequent addition of mononucleotides to the growing chain was performed after selective removal of the 5'-monomethoxytrityl group. Dicyclohexylcarbodiimide (DCC) was used as condensing reagent throughout the synthesis. The excess of mononucleotide and the yield in each addition reaction were as follows : UpGpG (protected) 2.2 fold, 28%, GpUpGpG (protected), 5 fold, 32% ; GpGpUpGpG (protected) 10 fold, 35% ; GpGpUpGpG, 10 fold, 12%. The protected intermediate oligomers were isolated by ion-exchange chromatography on TEAE-cellulose columns and identified by enzymatic hydrolyses after deblocking. The hexanucleotide was separated by gel filtration on Sephadex LH-20 and purified on DEAE-cellulose in the presence of 7 M urea at 55°. The circular dichroism spectra of these guanine rich ribooligomers have measured and a marked difference between the pentamer GpGpUpGpG and the hexamer GpGpUpGpG has been observed.
- 公益社団法人日本薬学会の論文
- 1978-10-25
著者
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田中 俊樹
Faculty Of Pharmaceutical Sciences Osaka University:(present Address)protein Engineering Research In
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池原 森男
Faculty of Pharmaceutical Sciences, Osaka University(Present address)
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大塚 栄子
Faculty Of Pharmaceutical Sciences Hokkaido University
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中川 栄子
Faculty of Pharmaceutical Sciences, Osaka University
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MARKHAM ALEXANDERF.
Faculty of Pharmaceutical Sciences, Osaka University
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Markham Alexanderf.
Faculty Of Pharmaceutical Sciences Osaka University
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中川 栄子
Faculty Of Pharmaceutical Sciences Osaka University
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池原 森男
Faculty Of Pharmaceutical Sciences Osaka University
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