Chemical Synthesis of Deoxyribonucleotides Containing Deoxyadenosine at the 3'-End on a Polystyrene Polymer Support

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概要

• 論文の詳細を見る

• Deoxyribonucleotides containing 2'-deoxyadenosine at the 3'-terminal, i.e., a heptadeoxyribonucleotide, d-GATTCTA, two pentadecadeoxyribonucleotides, d-A_<15>, d-AATGAGAAGCAACGA and a nonadecadeoxyribonucleotide, d-AGAATCTCGTTGCTTCTCA, were synthesized on a polystyrene polymer support by using the phosphotriester method. Dimer blocks (3) were coupled for elongation in the 5'-direction by using 1-mesitylenesulfonyl-3-nitro-1H-1,2,4-triazole as the activating reagent. A 3% trichloroacetic acid solution in dichloromethane was used for the detritylation process without causing any detectable depurination. 5'-O-Phosphorylated heptanucleotide and pentadecanucleotide were successfully joined by using deoxyribonucleic acid ligase in the presence of a template.

• 公益社団法人日本薬学会の論文
• 1985-12-25

著者

• 田中 俊樹

  Faculty Of Pharmaceutical Sciences Osaka University:(present Address)protein Engineering Research In

• 大石 武

  Faculty Of Engineering Yokohama National University:(present Address)meiji College Of Pharmacy

• 大石 武

  Meiji College Of Pharmacy

• 大石 武

  The Institute of Physical and Chemical Research (Riken)

• 田中 俊樹

  The Institute of Physical and Chemical Research (Riken).

• 大石 武

  The Institute Of Physical And Chemical Research (riken).

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