Sporolactobacillus inulinusのビタミンB_1要求性

概要

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Sporolactobacillus inulinus has been known to require essentially valine, leucine, biotin and calcium pantothenate and secondarily glutamic acid. But, this organism can not grow in the medium containing glucose and minerals in addition to the above mentioned nutrients. In an attempt to prepare the minimum growth medium, effects of amino acids and vitamins on the growth of the bacteria were investigated in details. A minute quantity of thiamine, 2.5 ng/ml, was known to be essential. The reason of misunderstanding in the previous paper is considered to result from contamination from the natural medium (GYP). Supplementary effect of folic acid or p-aminobenzoic acid (PABA) was also found. Thus the substantial composition of the basal minimum medium was determined as : glucose, sodium acetate, minerals, valine, leucine, glutamic acid, biotin, calcium pantothenate, PABA and thiamine.
日本ビタミン学会の論文
1977-03-25