Measurement of viable animal cell concentration based on fluorometry.
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概要
- 論文の詳細を見る
In viable animal cells, fluorescein diacetate is hydrolyzed to fluorescein (a fluorescent compound) which is retained in the cells. Fluorescein was effectively extracted from the cells by treatment with hypotonic phosphate buffer (1 mmol·dm<SUP>–3</SUP>, pH 7.4) and the extract showed a fluorescent peak at excitation and emission wavelengths of 496.5 and 515 nm, respectively. A linear relationship was confirmed between the viable cell concentrations based on fluorometry and microscopy. Animal cell growth could be successfully monitored based on fluorometry for suspension and microcarrier cultures of animal cells.
- The Society of Chemical Engineers, Japanの論文
著者
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Kobayashi Takeshi
Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University
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Taya Masahito
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Yoshikawa Munetaka
Department Of Biotechnology Faculty Of Engineering Osaka University
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Yoshikawa Munetaka
Department of Chemical Engineering, Nagoya University
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