マイクロプレートを用いた酸性プロテアーゼ活性の新規測定法

概要

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A novel determination method of acidic-protease was developed by using casein-gellan gum gel in a microplate. Acidic casein solution (in 0.72% lactic acid, pH2.6) was mixed with gellan gum at 60℃ and solidified in wells of a microplate. Casein micelle was dispersed in the gellan gum gel as colloidal perticle. By adding pepsin as a standard acidic endo-protease on the well, OD_<655> of the casein dispersed gel was decreased by the pepsin action. The intensity of the pepsin activity was in logarithmic proportion to regression coefficient of the reaction degree and the reaction time. The results of acidic protease activity determined by present method gave good agreement with the values obtained by the conventional method. By using present method, it may be possible to determine quick acidic-protease activity and/or multi-sample treatment by an automatic devise.
日本食品化学学会の論文
1998-11-30