Aerobacter aerogenesによるisoamylaseの生産 : (I)窒素源の影響

概要

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For the purpose of industrial production and application of isoamylase, the authors carried out screening tests, and as a result, a strain of Aerobacter aerogenes was isolated. Isoamylase production by this strain was investigated by means of shake culture. When a soluble starch as a carbon source, and CH_3COONH_4 as nitrogen source. were used, isoamylase activity in the culture filtrate was about 500 units per ml after 100 hrs. of cultivation. While, in a medium containing the same carbon source and (NH_4)_2SO_4 instead of CH_3COONH_4 as the carbon source, the isoamylase formation and the cell growth were proceeded simultaneously, but almost all isoamylase formed was a cell-bound type after 18 hrs. of cultivation. Its activity was analyzed to be 120 units per ml of the culture medium (27 units per mg of dry cell weight). So, the isolated strain was considered to hold two different isoamylase formation mechanisms representing either way according to the type of the nitrogen source used. As to the possibility of industrial production, the authors reached a conclusion that the isolate may be a good strain for industrial use, because the cell bound type enzymes and the cells were produced both quantitatively and simultaneously and also -because of the high productivity of isoamylase per unit time and per unit culture medium.
社団法人日本生物工学会の論文